Human ITGA2 (CD49b) knockout HeLa cell lysate
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ITGA2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 4.
View Alternative Names
BR, CD 49b, CD49 antigen-like family member B, Collagen Receptor, GPla, HPA 5, Human platelet alloantigen system 5, ITGA2, Integrin alpha-2, Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA 2 receptor), Platelet antigen Br, Platelet glycoprotein GPIa, Platelet glycoprotein Ia, Platelet membrane glycoprotein 1a, Platelet membrane glycoprotein Ia, VLA 2, VLA 2 alpha chain, VLA-2 subunit alpha, VLA2 receptor, alpha 2 subunit, VLAA2, Very late activation protein 2 receptor alpha 2 subunit
- WB
Lab
Western blot - Human ITGA2 (CD49b) knockout HeLa cell lysate (AB257262)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : ITGA2 knockout HeLa cell lysate (20 μg)
Lane 3 : A431 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab181549 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.
ab181549 Anti-Integrin alpha 2 antibody [EPR17349] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264819 (knockout cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab181549 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Integrin alpha 2 antibody [EPR17349] (<a href='/en-us/products/primary-antibodies/integrin-alpha-2-antibody-epr17349-ab181549'>ab181549</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ITGA2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ITGA2 (CD49b) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-itga2-cd49b-knockout-hela-cell-line-ab264819'>ab264819</a>)
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
K562 cell lysate at 20 µg
Predicted band size: 129 kDa,130 kDa,171 kDa
Observed band size: 160 kDa,250 kDa
false
- WB
Lab
Western blot - Human ITGA2 (CD49b) knockout HeLa cell lysate (AB257262)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : ITGA2 knockout HeLa cell lysate (20μg)
Lane 3 : A431 cell lysate (20μg)
Lane 4 : K562 cell lysate (20μg)
Lanes 1- 4 : Merged signal (red and green). Green - ab109432 observed at 129 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109432 Anti-ITGA2 antibody [epr5789] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout sample ab260092 was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (<a href='/en-us/products/primary-antibodies/integrin-alpha-2-antibody-epr5789-ab109432'>ab109432</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ITGA2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ITGA2 (CD49b) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-itga2-cd49b-knockout-hela-cell-line-ab264819'>ab264819</a>)
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
K562 cell lysate at 20 µg
Predicted band size: 129 kDa,130 kDa
Observed band size: 129 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human ITGA2 (CD49b) knockout HeLa cell lysate (AB257262)
Homozygous : 14 bp deletion in exon 4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The CD49b protein plays a critical role in cell migration thrombosis and wound healing. By forming a heterodimer with Integrin beta-1 it becomes a part of the collagen receptor complex Integrin alpha-2/beta-1 which facilitates various cellular interactions with the ECM. This functionality enables cells to sense their environment and respond to mechanical changes which is important for processes such as tissue repair and embryonic development.
Pathways
Several cellular mechanisms involve CD49b in maintaining physiological homeostasis. One significant pathway is the integrin signaling pathway where CD49b interacts with focal adhesion kinase (FAK) and other intracellular proteins to regulate cytoskeletal rearrangement and cell motility. Another pathway is platelet activation where CD49b collaborates with glycoprotein VI (GPVI) and other receptors to initiate platelet aggregation during hemostasis. These pathways highlight the importance of CD49b in various cellular functions related to cell mobility and stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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