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AB273764

Human ITGA6 (Integrin alpha 6) knockout SW480 cell lysate

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ITGA6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%.
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Next Generation Sequencing - Human ITGA6 (Integrin alpha 6) knockout SW480 cell lysate (AB273764)
  • NGS

Supplier Data

Next Generation Sequencing - Human ITGA6 (Integrin alpha 6) knockout SW480 cell lysate (AB273764)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99%

Key facts

Cell type

SW480

Species or organism

Human

Tissue

Colon

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%

Disease

Adenocarcinoma

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Primary Antibodies

AB181551

Anti-Integrin alpha 6 antibody [EPR18124]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (AB181551)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ITGA6
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6a often referred to as ITGA6 is a part of the integrin protein family. This protein acts mechanically as a receptor that binds extracellular matrix components. It functions mainly in cell adhesion and signal transduction processes. Integrin alpha 6a typically forms a dimer with the beta 1 or beta 4 subunit. This protein has a mass of approximately 130 kDa. Researchers observe its expression in epithelial cells endothelial cells and some immune cells throughout various tissues.
Biological function summary

Integrin alpha 6a plays a significant role in cell-matrix interactions by binding to laminin a major component of the basement membrane. It is part of a larger integrin complex known to affect cellular processes such as migration proliferation and differentiation. By mediating these interactions Integrin alpha 6a influences the structural stability of tissues and facilitates responses to cellular environment changes.

Pathways

Integrin alpha 6a participates in the MAPK and PI3K/Akt signaling pathways. These pathways are important for various cellular processes including growth survival and differentiation. Integrin alpha 6a associates with related integrin proteins such as Integrin beta 1 in these pathways where it modulates downstream signaling cascades that regulate cellular behavior and responses.

Integrin alpha 6a has associations with cancers and epidermolysis bullosa. Its expression correlates with tumor progression and metastasis particularly in cancers like breast and prostate. Integrin alpha 6a's involvement in epidermolysis bullosa links it to defects in epithelial-dermal cohesion. In these contexts its interaction with laminin and other integrins contributes significantly to pathogenesis and progression of these conditions.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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