ITGA6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%.
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Key facts
- Cell type
- SW480
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%
Alternative names
CD49 antigen-like family member F, CD49f, ITA6_HUMAN, ITGA6, Integrin alpha 6, Integrin alpha chain alpha 6, Integrin alpha-6 light chain, VLA-6
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ITGA6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%.
Key facts
- Cell type
- SW480
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- ITGA6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing, Western blot
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 6a often referred to as ITGA6 is a part of the integrin protein family. This protein acts mechanically as a receptor that binds extracellular matrix components. It functions mainly in cell adhesion and signal transduction processes. Integrin alpha 6a typically forms a dimer with the beta 1 or beta 4 subunit. This protein has a mass of approximately 130 kDa. Researchers observe its expression in epithelial cells endothelial cells and some immune cells throughout various tissues.
- Biological function summary
Integrin alpha 6a plays a significant role in cell-matrix interactions by binding to laminin a major component of the basement membrane. It is part of a larger integrin complex known to affect cellular processes such as migration proliferation and differentiation. By mediating these interactions Integrin alpha 6a influences the structural stability of tissues and facilitates responses to cellular environment changes.
- Pathways
Integrin alpha 6a participates in the MAPK and PI3K/Akt signaling pathways. These pathways are important for various cellular processes including growth survival and differentiation. Integrin alpha 6a associates with related integrin proteins such as Integrin beta 1 in these pathways where it modulates downstream signaling cascades that regulate cellular behavior and responses.
- Associated diseases and disorders
Integrin alpha 6a has associations with cancers and epidermolysis bullosa. Its expression correlates with tumor progression and metastasis particularly in cancers like breast and prostate. Integrin alpha 6a's involvement in epidermolysis bullosa links it to defects in epithelial-dermal cohesion. In these contexts its interaction with laminin and other integrins contributes significantly to pathogenesis and progression of these conditions.
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Next Generation Sequencing - Human ITGA6 (Integrin alpha 6) knockout SW480 cell lysate (ab273764)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99%
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