Human ITPA knockout HEK-293T cell lysate
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ITPA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
C20orf37, HLC14-06-P, ITPA_HUMAN, ITPase, Inosine triphosphatase, Inosine triphosphatase (nucleoside triphosphate pyrophosphatase), Inosine triphosphate pyrophosphatase, Inosine triphosphate pyrophosphohydrolase, My049, My049 protein, NTPase, Non canonical purine NTP pyrophosphatase, Non standard purine NTP pyrophosphatase, Nucleoside triphosphate pyrophosphatase, OK/SW-cl.9, OTTHUMP00000030094, OTTHUMP00000160459, Putative oncogene protein hlc14-06-p, dJ794I6.3, inosine triphosphatase-A, nucleoside triphosphate diphosphatase
- WB
Lab
Western blot - Human ITPA knockout HEK-293T cell lysate (AB258477)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ITPA knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
ab150420 was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab150420 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ITPA antibody [EPR8779] (<a href='/en-us/products/primary-antibodies/itpa-antibody-epr8779-ab150420'>ab150420</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
ITPA knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human ITPA knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-itpa-knockout-hek-293t-cell-line-ab266679'>ab266679</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ITPA knockout HEK-293T cell lysate (AB258477)
Homozygous : 1 bp insertion in exon 1
- WB
Lab
Western blot - Human ITPA knockout HEK-293T cell lysate (AB258477)
Lanes 1-3 : Merged signal (red and green). Green - ab134937 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.
ab134937 Anti-ITPA antibody [EPR8780] was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab134937 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ITPA antibody [EPR8780] (<a href='/en-us/products/primary-antibodies/itpa-antibody-epr8780-ab134937'>ab134937</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human ITPA knockout HEK-293T cell lysate (ab258477) at 20 µg
Lane 3:
Jurkat cell lysate at 20 µg
Secondary
Lanes 1 - 3:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Lanes 1 - 3:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme plays a pivotal role in nucleotide metabolism by regulating the levels of ITP deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP). By hydrolyzing these non-standard nucleotides ITPA maintains genomic integrity and prevents mutations during DNA replication. It is not typically considered part of a larger complex but acts independently to safeguard the nucleotide pool from potentially harmful nucleotide analogs.
Pathways
ITPA's activity is essential in the purine metabolism pathway where it helps maintain a proper balance of nucleotide synthesis and degradation. This function helps prevent the incorporation of non-standard bases into DNA and RNA which can lead to genetic instability. ITPA activity is also intertwined with the salvage pathway of purine nucleotides. This connection highlights its indirect relationship with proteins involved in nucleotide synthesis and repair such as DNA polymerases and other nucleotide-metabolizing enzymes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-ITPA antibody [EPR8780] (AB134937)](https://content.abcam.com/products/images/itpa-antibody-epr8780-ab134937--western-blot-img346.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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