Human IWS1 knockout HeLa cell lysate
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IWS1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
View Alternative Names
IWS1-like protein, IWS1L, Protein IWS1 homolog, IWS1
- Sanger seq
Unknown
Sanger Sequencing - Human IWS1 knockout HeLa cell lysate (AB263237)
Homozygous : Insertion of the selection cassette in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The IWS1 protein is involved in mediating interactions between the transcription machinery and chromatin. IWS1 often functions as a part of the transcription elongation complex enhancing the efficiency of RNA polymerase II during the elongation phase of transcription. IWS1 as part of this complex assists in chromatin remodeling which allows for the transcription of tightly packaged DNA. This action facilitates efficient gene expression and is important in maintaining proper cellular functions and responses to environmental signals.
Pathways
IWS1 integrates into critical cellular processes like the cell cycle and DNA damage response pathways. One significant pathway is the RNA polymerase II transcription elongation pathway where IWS1 works closely with other elongation factors and chromatin remodeling proteins to ensure proper transcription regulation. Additionally IWS1 is associated with the DNA damage response pathway where it collaborates with proteins such as ATR and BRCA1 to manage cellular reactions to DNA damage contributing to genome stability and repair mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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