Human JAK2 knockout A549 cell lysate
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- WB
Lab
Western blot - Human JAK2 knockout A549 cell lysate (AB256963)
Lane 1 : Wild-Type A549 cell lysate (20µg)
Lane 2 : JAK2 knockout A549 cell lysate (20µg)
Lane 3 : K562 cell lysate (20µg)
Lane 4 : Daudi cell lysate (20µg)
Lanes 1- 4 : Merged signal (red and green). Green - ab108596 observed at 131 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab108596 Anti-JAK2 antibody [EPR108(2)] was shown to specifically react with JAK2 in Wild-Type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267113 (knockout cell lysate ab256963) was used. Wild-type and JAK2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108596 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-JAK2 antibody [EPR108(2)] (<a href='/en-us/products/primary-antibodies/jak2-antibody-epr1082-ab108596'>ab108596</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
JAK2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human JAK2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-jak2-knockout-a549-cell-line-ab267113'>ab267113</a>)
Lane 3:
K562 cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Predicted band size: 131 kDa
Observed band size: 131 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human JAK2 knockout A549 cell lysate (AB256963)
Allele-1 : 5 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human JAK2 knockout A549 cell lysate (AB256963)
Allele-2 : 1 bp insertion in exon3
Complementary Products
Primary Antibodies
AB108596
Anti-JAK2 antibody [EPR108(2)]
20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size|Trial Size
![Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (AB108596)](https://content.abcam.com/products/images/jak2-antibody-epr1082-ab108596--immunocytochemistry-immunofluorescence-img14624.jpg)
- 4
- 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.
Pathways
The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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