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AB257490

Human JMJD6 knockout HEK-293T cell lysate

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JMJD6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

View Alternative Names

Apoptotic cell clearance receptor, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, Histone arginine demethylase JMJD6, JMJD6_HUMAN, JmjC domain-containing protein 6, Jumonji domain containing 6, Jumonji domain-containing protein 6, KIAA0585, Lysyl-hydroxylase JMJD6, PSR, PTDSR, PTDSR 1, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR

3 Images
Western blot - Human JMJD6 knockout HEK-293T cell lysate (AB257490)
  • WB

Lab

Western blot - Human JMJD6 knockout HEK-293T cell lysate (AB257490)

Lane 1 : Wild-type HEK293T cell lysate 20 ug
Lane 2 : JMJD6 knockout HEK293T cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab64575 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab64575 was shown to react with JMJD6 in wild-type HEK293T cells in Western blot with loss of signal observed in JMJD6 knockout cell line ab266402 (JMJD6 knockout cell lysate ab257490). Wild-type and JMJD6 knockout HEK293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab64575 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-JMJD6 antibody (<a href='/en-us/products/primary-antibodies/jmjd6-antibody-ab64575'>ab64575</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

JMJD6 Knockout HEK293T cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 50 kDa

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Western blot - Human JMJD6 knockout HEK-293T cell lysate (AB257490)
  • WB

Lab

Western blot - Human JMJD6 knockout HEK-293T cell lysate (AB257490)

Lane 1 : THP-1 cell lysate (20 ug)

Lane 2 : Wild-type HEK293T cell lysate (20 ug)

Lane 3 : JMJD6 knockout HEK293T cell lysate (20 ug)

Lanes 1 - 3 : Merged signal (red and green). Green - ab64575 observed at 50 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab64575 was shown to react with JMJD6 in wild-type HEK-293 cells in western blot. Loss of signal was observed when JMJD6 knockout cell lysate (ab257490) was used. Wild-type and JMJD6 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab64575 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-JMJD6 antibody (<a href='/en-us/products/primary-antibodies/jmjd6-antibody-ab64575'>ab64575</a>) at 1 µg/mL

Lane 1:

THP1 cell lysate at 20 µg

Lane 2:

Wild-type HEK293 cell lysate at 20 µg

Lane 3:

JMJD6 knockout HEK293 cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 50 kDa

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Sanger Sequencing - Human JMJD6 knockout HEK-293T cell lysate (AB257490)
  • Sanger seq

Unknown

Sanger Sequencing - Human JMJD6 knockout HEK-293T cell lysate (AB257490)

Homozygous : 1 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
JMJD6
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JMJD6 also known as Jumonji Domain Containing 6 or PSR (Phosphatidylserine Receptor) is a protein with a molecular mass of approximately 47 kDa. It functions mechanically as a 2-oxoglutarate- and Fe(II)-dependent oxygenase. This protein resides in the nucleus and cytoplasm with a higher expression in the thymus kidney heart and testis. JMJD6 gets involved in demethylating histones and splicing factors playing a role in gene regulation.
Biological function summary

JMJD6 influences gene expression and mRNA splicing processes. It participates in histone arginine demethylation affecting chromatin structure and transcriptional activity. JMJD6 is not known to be part of a larger protein complex but interacts with RNA splicing factors and chromatin modifiers. Its demethylase activity can alter the epigenetic landscape which makes it a vital part of cellular response and differentiation processes.

Pathways

JMJD6 is linked to the regulation of gene expression and RNA processing pathways. It modulates the transcriptional networks by its demethylase activity affecting how genes are turned on or off. JMJD6 often associates with nuclear receptors and splicing proteins such as SF3B1 integrating within the larger network of gene expression controllers and RNA splicing machinery. These interactions place JMJD6 at a critical juncture in gene expression regulation and RNA maturation.

JMJD6 has connections with cancer and autoimmune diseases. Its role in gene regulation and splicing implicates it in cancer progression often in link with prostate cancer. Abnormal JMJD6 expression can lead to disrupted gene patterns contributing to tumor growth. Additionally its alteration has associations with autoimmune diseases where its interaction with histone-modifying proteins like HDAC affects the immune response. These relationships highlight JMJD6's involvement in disease states marking it as a target of interest in research for therapeutic interventions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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