Human JOSD1 knockout HEK-293T cell lysate
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JOSD1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
JOS1_HUMAN, Josephin domain-containing 1, Josephin-1, KIAA0063, OTTHUMP00000198984, OTTHUMP00000239365, dJ508I15.2
- Sanger seq
Unknown
Sanger Sequencing - Human JOSD1 knockout HEK-293T cell lysate (AB258930)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein modifies the stability and activity of other proteins by removing ubiquitin molecules. It acts as part of a larger deubiquitinase complex which interacts with protein networks to maintain cellular homeostasis. Through its enzyme activity JOSD1 contributes to the regulation of processes such as protein quality control and cell cycle progression.
Pathways
Scientists have placed JOSD1 in the ubiquitin-proteasome system an important pathway responsible for protein turnover in cells. It has connections with other proteins like USP7 and USP9X in this pathway reflecting its coordination role in protein deubiquitination. JOSD1 also participates in the endoplasmic reticulum-associated degradation (ERAD) pathway helping to eliminate misfolded proteins important for maintaining protein folding homeostasis within the cell.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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