Human KDELR2 (ELP-1) knockout HEK-293T cell lysate
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KDELR2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
(Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2, ER lumen protein retaining receptor 2, ERD 2 like protein, ERD2-like protein 1, ERD2.2, ERD22_HUMAN, FLJ45532, KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2, KDEL endoplasmic reticulum protein retention receptor 2, KDEL receptor 2, KDELR 2
- Sanger seq
Unknown
Sanger Sequencing - Human KDELR2 (ELP-1) knockout HEK-293T cell lysate (AB258482)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ELP-1 plays an important role in facilitating the modification of tRNA wobble uridines. This allows for accurate and efficient translation of the genetic code into proteins. ELP-1 as a vital part of the Elongator complex also contributes to the acetylation of histones which impacts chromatin structure and gene expression regulation. The protein ensures that gene transcription proceeds effectively by interacting with different components of the transcription machinery.
Pathways
The Elongator complex including ELP-1 integrates into the transcriptional regulation and tRNA modification pathways. ELP-1 interacts with several proteins like RNA polymerase II during gene transcription. Another intersecting pathway includes the regulation of response to cellular stress as correct modification of tRNA maintains protein synthesis under stress conditions. Its functionality within these pathways illustrates its integral role in ensuring proper cellular response to environmental changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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