Human KDM1A (LSD1) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
KDM1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon1.
View Alternative Names
AOF2, Amine oxidase (flavin containing) domain 2, Amine oxidase, flavin containing, 2, BHC110, BRAF35-HDAC complex protein BHC110, BRAF35/HDAC complex, 110-kD subunit, CPRF, EC1, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, KDM 1, KDM1A_HUMAN, KIAA0601, LSD 1, Lysine (K) specific demethylase 1, Lysine (K) specific demethylase 1A, Lysine demethylase 1A, Lysine-specific demethylase 1, Lysine-specific demethylase 1A, Lysine-specific histone demethylase 1, Lysine-specific histone demethylase 1A
- WB
Lab
Western blot - Human KDM1A (LSD1) knockout HeLa cell lysate (AB256965)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : KDM1A knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab129195 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.
ab129195 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker was shown to specifically react with KDM1/LSD1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265790 (knockout cell lysate ab256965) was used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129195 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (<a href='/en-us/products/primary-antibodies/kdm1-lsd1-antibody-epr6825-nuclear-marker-and-chip-grade-ab129195'>ab129195</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
KDM1A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-kdm1a-lsd1-knockout-hela-cell-line-ab265790'>ab265790</a>)
Predicted band size: 155 kDa,92 kDa
Observed band size: 110 kDa,160 kDa
false
- WB
Lab
Western blot - Human KDM1A (LSD1) knockout HeLa cell lysate (AB256965)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : KDM1A knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab69535 observed at 110 kDa. Red - loading control ab181602 observed at 37 kDa.
ab69535 Anti-KDM1/LSD1 antibody [1B2F2] was shown to specifically react with KDM1/LSD1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265790 (knockout cell lysate ab256965) was used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab69535 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KDM1/LSD1 antibody [1B2F2] (<a href='/en-us/products/primary-antibodies/kdm1-lsd1-antibody-1b2f2-ab69535'>ab69535</a>)
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
KDM1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-kdm1a-lsd1-knockout-hela-cell-line-ab265790'>ab265790</a>)
Predicted band size: 92 kDa
Observed band size: 110 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human KDM1A (LSD1) knockout HeLa cell lysate (AB256965)
Homozygous : 4 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB17721
Anti-KDM1/LSD1 antibody - Nuclear Marker
KO Validated|Lab Essentials
- 4
- 25
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com