Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate
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KDM1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon3 and 5 bp deletion in exon3.
View Alternative Names
AOF1, Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, KDM1B, C6orf193, Flavin-containing amine oxidase domain-containing protein 1, LSD2
- WB
Lab
Western blot - Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate (AB258016)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : KDM1B knockout HeLa cell lysate (20 ug)
Lane 3 : HAP1 cell lysate (20 ug)
Lane 4 : HCT116 cell lysate (20 ug)
ab193080 was shown to specifically react with LSD2 / AOF1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265969 (knockout cell lysate ab258016) was used. Wild-type and LSD2 / AOF1 knockout samples were subjected to SDS-PAGE. ab193080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-LSD2 / AOF1 antibody [EPR18508] (<a href='/en-us/products/primary-antibodies/lsd2-aof1-antibody-epr18508-ab193080'>ab193080</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
KDM1B knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human KDM1B (LSD2 / AOF1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-kdm1b-lsd2-aof1-knockout-hela-cell-line-ab265969'>ab265969</a>)
Lane 3:
HAP1 cell lysate at 20 µg
Lane 4:
HCT116 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 95 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate (AB258016)
Allele-1 : 5 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate (AB258016)
Allele-2 : 2 bp deletion in exon3
Complementary Products
Primary Antibodies
AB193080
Anti-LSD2 / AOF1 antibody [EPR18508]
RabMAb|Recombinant|KO Validated|20ul selling size
![Flow Cytometry (Intracellular) - Anti-LSD2 / AOF1 antibody [EPR18508] (AB193080)](https://content.abcam.com/products/images/lsd2-aof1-antibody-epr18508-ab193080--flow-cytometry-intracellular-img13741.jpg)
- 0
- 0
Proteins & Peptides
AB271603
Recombinant human LSD2 / AOF1 protein (Active) (GST N-Terminus + TEV N-Terminus)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LSD2 impacts gene expression by altering the epigenetic state of chromatin. It is not part of a well-defined large multiprotein complex allowing LSD2 to directly interact with histones. Through its demethylase activity it modulates the transcriptional regulation impacting cellular differentiation and proliferation. This makes it an important factor in maintaining cellular homeostasis.
Pathways
LSD2 is involved in histone modification processes particularly through the demethylation of H3K4me1 and H3K4me2 marks. These changes are important in the regulation of gene expression patterns. LSD2 often interacts with the REST corepressor complex affecting the expression of genes involved in neural development and function. It plays a role in pathways related to cellular stress responses and metabolic regulation highlighting its importance in cellular adaptation mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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