Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate
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KDM5C KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon4 and 5 bp deletion in exon4.
View Alternative Names
DXS1272E, Histone demethylase JARID1C, JARID1C, JmjC domain containing protein SMCX, Jumonji AT rich interactive domain 1C, Jumonji, AT rich interactive domain 1C (RBP2 like), Jumonji/ARID domain-containing protein 1C, KDM5C_HUMAN, Lysine (K) specific demethylase 5C, Lysine-specific demethylase 5C, MRX13, MRXJ, MRXSCJ, MRXSJ, Protein SmcX, Protein Xe169, SMCX, Selected cDNA on X, SmcX protein, Smcx homolog X chromosome, Smcy homolog X linked, XE169, Xe169 protein, rbp2 like protein
- WB
Lab
Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (AB257495)
Blocking and Diluting buffer and concentration : 5% NFDM/TBST
Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate, 20 ug
Lane 2 : ab266252, KDM5C knockout HEK293T (human embryonic kidney epithelial cell, whole cell lysate, 20 ug
Lane 3 : ab266251, KDM5C knockout HEK293T (human embryonic kidney epithelial cell), whole cell lysate, 20 ug
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, 20 ug
Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 2:
Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266252'>ab266252</a>) at 20 µg
Lane 3:
Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg
Lane 4:
HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>)
Predicted band size: 175 kDa
Observed band size: 180 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (AB257495)
Allele-1 : 17 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell lysate (AB257495)
Allele-2 : 5 bp deletion in exon4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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