Human KIF22 knockout HEK-293T cell lysate
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KIF22 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
View Alternative Names
A 328A3.2, KID, KIF22_HUMAN, KNSL 4, Kinesin family member 22, Kinesin like 4, Kinesin like DNA binding protein pseudogene, Kinesin-like DNA-binding protein, Kinesin-like protein 4, Kinesin-like protein KIF22, OBP, OBP 1, OBP 2, OTTHUMP00000123406, OriP binding protein, Origin of plasmid DNA replication binding protein, SEMDJL2
- WB
Lab
Western blot - Human KIF22 knockout HEK-293T cell lysate (AB258018)
This image was generated using the hybridoma version of this product.
Lanes 1 - 2 : Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75783 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KIF22 antibody [EP2747Y] (<a href='/en-us/products/primary-antibodies/kif22-antibody-ep2747y-ab75783'>ab75783</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
KIF22 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human KIF22 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kif22-knockout-hek-293t-cell-line-ab266817'>ab266817</a>)
Predicted band size: 73 kDa
Observed band size: 75 kDa
false
- WB
Lab
Western blot - Human KIF22 knockout HEK-293T cell lysate (AB258018)
Lane 1 : Wild-type HEK-293T cell lysate 20 ug
Lane 2 : KIF22 knockout HEK-293T cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab108535 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108535 was shown to react with KIF22 in wild-type HEK-293T cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively.. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KIF22 antibody [EP2748] (<a href='/en-us/products/primary-antibodies/kif22-antibody-ep2748-ab108535'>ab108535</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
KIF22 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human KIF22 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kif22-knockout-hek-293t-cell-line-ab266817'>ab266817</a>)
Predicted band size: 107 kDa,130 kDa,22 kDa,33 kDa,34 kDa,38 kDa,42 kDa,43 kDa,60 kDa,73 kDa,96 kDa
Observed band size: 107 kDa,17 kDa,33 kDa,34 kDa,42 kDa,70 kDa,75 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human KIF22 knockout HEK-293T cell lysate (AB258018)
Homozygous : 1 bp insertion in exon 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)](https://content.abcam.com/products/images/kif22-antibody-ep2747y-ab75783--western-blot-img147025.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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