Human KNSTRN (SKAP) knockout HEK-293T cell lysate
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KNSTRN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
C15orf23, HSD 11, KNSTRN, Kinastrin, Kinetochore-localized astrin-binding protein, Kinetochore-localized astrin/SPAG5-binding protein, Putative TRAF4-associated factor 1, T4AF1_HUMAN, TRAF4-associated factor 1, Traf4af1, chromosome 15 open reading frame 23, small kinetochore associated protein
- Sanger seq
Unknown
Sanger Sequencing - Human KNSTRN (SKAP) knockout HEK-293T cell lysate (AB258934)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human KNSTRN (SKAP) knockout HEK-293T cell lysate (AB258934)
Allele-1 : 4 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SKAP regulates immune cell function by influencing adhesion and migration processes. It often forms part of larger complexes within the immune synapse facilitating interactions between proteins necessary for efficient signaling. SKAP's role in T cells involves partnering with other signaling molecules to strengthen immune responses against infections by promoting cell adhesion.
Pathways
SKAP participates actively in the T-cell receptor (TCR) signaling and integrin-mediated pathways. It associates with proteins such as LFA-1 an important integrin that mediates cell adhesion. SKAP affects the downstream signaling events by modulating key interactions and conformations which are important for effective immune response initiation and regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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