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KRT14 KO cell lysate available now. KO validated by Immunocytochemistry, Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 13 bp deletion Frameshift = 99.9%.

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Images

Western blot - Human KRT14 (Cytokeratin 14) knockout A-431 cell lysate (AB261706), expandable thumbnail
  • Next Generation Sequencing - Human KRT14 (Cytokeratin 14) knockout A-431 cell lysate (AB261706), expandable thumbnail
  • Western blot - Human KRT14 (Cytokeratin 14) knockout A-431 cell lysate (AB261706), expandable thumbnail
  • Western blot - Human KRT14 (Cytokeratin 14) knockout A-431 cell lysate (AB261706), expandable thumbnail

Key facts

Cell type
A-431
Species or organism
Human
Tissue
Skin
Knockout validation
Immunocytochemistry, Next Generation Sequencing, Western blot
Mutation description
Knockout achieved by CRISPR/Cas9 X = 13 bp deletion Frameshift = 99.9%

Alternative names

CK-14, Cytokeratin-14, Dowling Meara, EBS3, EBS4, Epidermolysis bullosa simplex, K14, K1C14_HUMAN, Keratin, Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), Keratin type I cytoskeletal 14, Keratin-14, Koebner, Krt 14, NFJ, OTTHUMP00000164624, type I cytoskeletal 14

What's included?

1 Kit
Components
Human KRT14 knockout A431 cell lysate
1 x 100 µg
Human wild-type A-431 cell lysate
1 x 100 µg

Recommended products

KRT14 KO cell lysate available now. KO validated by Immunocytochemistry, Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 13 bp deletion Frameshift = 99.9%.

Key facts

Cell type
A-431
Mutation description
Knockout achieved by CRISPR/Cas9 X = 13 bp deletion Frameshift = 99.9%
Disease
Epidermoid Carcinoma
Concentration
Loading...

Properties

Gene name
KRT14
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Immunocytochemistry, Next Generation Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Associated diseases and disorders

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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