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AB261676

Human KRT7 knockout A549 cell lysate

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KRT7 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 100%.
7 Images
Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

ab192079 was shown to specifically react with KRT7 in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab192079 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/20000 dilution for 1 hour at room temperature. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (<a href='/en-us/products/primary-antibodies/hrp-cytokeratin-7-antibody-epr1619y-cytoskeleton-marker-ab192079'>ab192079</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human KRT7 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-krt7-knockout-a549-cell-line-ab261867'>ab261867</a>) at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

ab209945 was shown to specifically react with KRT7 in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab209945 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/20000 dilution for 1 hour at room temperature. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-Cytokeratin 7 antibody [EPR17078] (<a href='/en-us/products/primary-antibodies/hrp-cytokeratin-7-antibody-epr17078-ab209945'>ab209945</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human KRT7 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-krt7-knockout-a549-cell-line-ab261867'>ab261867</a>) at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 51 kDa

false

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lanes 1 - 4 : Merged signal (red and green). Green - ab183344 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183344 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab183344 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/25 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [SP52] (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-sp52-ab183344'>ab183344</a>) at 1/25 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

Western blot - Human KRT7 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-krt7-knockout-a549-cell-line-ab261867'>ab261867</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 51 kDa

Observed band size: 50 kDa

false

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab68459 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab68459 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab68459 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (<a href='/en-us/products/primary-antibodies/cytokeratin-7-antibody-epr1619y-cytoskeleton-marker-ab68459'>ab68459</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human KRT7 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-krt7-knockout-a549-cell-line-ab261867'>ab261867</a>) at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 51 kDa

Observed band size: 50 kDa

false

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab119697 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab119697 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab119697 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/25 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-Cytokeratin 7 antibody [SP52] (<a href='/en-us/products/unavailable/cytokeratin-7-antibody-sp52-ab119697'>ab119697</a>) at 1/25 dilution

Lane 1:

Wild-type A549 whole cell lysate at 20 µg

Lane 2:

KRT7 knockout A549 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

false

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)
  • WB

Lab

Western blot - Human KRT7 knockout A549 cell lysate (AB261676)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab68460 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab68460 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cell line ab261867 (knockout cell lysate ab261676). Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab68460 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (FITC) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-fitc-ab6840'>ab6840</a>) at 1/10000 dilution

false

Next Generation Sequencing - Human KRT7 knockout A549 cell lysate (AB261676)
  • NGS

Lab

Next Generation Sequencing - Human KRT7 knockout A549 cell lysate (AB261676)

X = 2 bp deletion

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift = 100%

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB181598

Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (AB181598)

  • 5
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Proteins & Peptides

AB132933

Recombinant Human Cytokeratin 7 protein

SDS-PAGE - Recombinant Human Cytokeratin 7 protein (AB132933)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab261676-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human KRT7 knockout A549 cell lysate", "number":"AB261676-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB261676-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
KRT7
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com