Human L1CAM knockout HeLa cell lysate
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L1CAM KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 154 bp insertion in exon1.
View Alternative Names
Antigen identified by monoclonal antibody R1, CAML1, CD171, CD171 antigen, HSAS, HSAS1, Hyd, L1, L1 cell adhesion molecule, L1-NCAM, L1CAM_HUMAN, MIC5, N-CAM-L1, NILE, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, OTTHUMP00000025992
- WB
Unknown
Western blot - Human L1CAM knockout HeLa cell lysate (AB263786)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : L1CAM knockout HeLa cell lysate (20 μg)
Lanes 1 - 2 : Merged signal (red and green). Green - ab182407 observed at 220 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab182407 was shown to react with L1CAM in wild-type HeLa. Loss of signal was observed when knockout cell line ab255401 (knockout cell lysate ab263786) was used. Wild-type and L1CAM knockout samples were subjected to SDS-PAGE. ab182407 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4^°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-L1CAM antibody [EPR18998] (<a href='/en-us/products/primary-antibodies/l1cam-antibody-epr18998-ab182407'>ab182407</a>) at 1/5000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
L1CAM knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human L1CAM knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-l1cam-knockout-hela-cell-line-ab255401'>ab255401</a>)
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 124 kDa,220 kDa
false
- WB
Unknown
Western blot - Human L1CAM knockout HeLa cell lysate (AB263786)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : L1CAM knockout HeLa cell lysate (20 μg)
Lanes 1 - 2 : Merged signal (red and green). Green - ab208155 observed at 220 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab208155 was shown to react with L1CAM in wild-type HeLa. Loss of signal was observed when knockout cell line ab255401 (knockout cell lysate ab263786) was used. Wild-type and L1CAM knockout samples were subjected to SDS-PAGE. ab208155 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-L1CAM antibody [EPR18750] (<a href='/en-us/products/primary-antibodies/l1cam-antibody-epr18750-ab208155'>ab208155</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
L1CAM knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human L1CAM knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-l1cam-knockout-hela-cell-line-ab255401'>ab255401</a>)
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 124 kDa,220 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human L1CAM knockout HeLa cell lysate (AB263786)
Allele-1 : 154 bp insertion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.
Pathways
L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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