L1CAM KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%
Alternative names
Antigen identified by monoclonal antibody R1, CAML1, CD171, CD171 antigen, HSAS, HSAS1, Hyd, L1, L1 cell adhesion molecule, L1-NCAM, L1CAM_HUMAN, MIC5, N-CAM-L1, NILE, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, OTTHUMP00000025992
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L1CAM KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- L1CAM
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
- Biological function summary
L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.
- Pathways
L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.
- Associated diseases and disorders
L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
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3 product images
Western blot - Human L1CAM knockout HeLa cell lysate (ab273790)
Lane 1: Wild-type HeLa cell lysate 20 μg.
Lane 2: L1CAM knockout HeLa cell lysate 20 μg.
False colour image of Western blot: Anti-L1CAM antibody [EPR23241-224] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot Anti-L1CAM antibody [EPR23241-224] ab270455 was shown to bind specifically to L1CAM. A band was observed at 220 kDa in wild-type HeLa cell lysates with no signal observed at this size in L1CAM knockout cell line Human L1CAM knockout HeLa cell line ab273836 (knockout cell lysate ab273790). The band observed in the knockout lysate lane below 220 kDa is likely to represent a truncated form of L1CAM. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and L1CAM knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-L1CAM antibody [EPR23241-224] (Anti-L1CAM antibody [EPR23241-224] ab270455) at 1/1000 dilution
Lanes 1 and 3: Wild-type HeLa cell lysate at 20 µg
Lanes 2 and 4: L1CAM knockout HeLa cell lysate at 20 µg
Lane 5: A375 cell lysate at 20 µg
Lane 6: MOLT-4 cell lysate at 20 µg
Predicted band size: 140 kDa
Observed band size: 220 kDa
Western blot - Human L1CAM knockout HeLa cell lysate (ab273790)
Lane 1: Wild-type HeLa cell lysate 20 μg
Lane 2: L1CAM knockout HeLa cell lysate 20 μg
False colour image of Western blot: Anti-L1CAM antibody [EPR18750] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot Anti-L1CAM antibody [EPR18750] ab208155 was shown to bind specifically to L1CAM. A band was observed at 220 kDa in wild-type HeLa cell lysates with no signal observed at this size in L1CAM knockout cell line Human L1CAM knockout HeLa cell line ab273836 (knockout cell lysate ab273790). The band observed in the knockout lysate lane below 220 kDa is likely to represent a truncated form of L1CAM. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and L1CAM knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (Anti-L1CAM antibody [EPR18750] ab208155) at 1/1000 dilution
Lanes 1 and 3: Wild-type HeLa cell lysate
Lanes 2 and 4: L1CAM knockout HeLa cell lysate
Lane 5: A375 cell lysate
Lane 6: MOLT-4 cell lysate
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 220 kDa
Next Generation Sequencing - Human L1CAM knockout HeLa cell lysate (ab273790)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift: 96%
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