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AB257499

Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate

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LAMB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

View Alternative Names

CLM, Cutis laxa with marfanoid phenotype, LAMB1_HUMAN, LIS5, Laminin B1, Laminin B1 chain, Laminin beta 1 chain, Laminin beta 1 chain precursor, Laminin beta1, Laminin subunit beta-1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

3 Images
Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)
  • WB

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Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : LAMB1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab108536 observed at 200 kDa. Red - loading control ab7291 observed at 50 kDa.

ab108536 Anti-Laminin beta 1 antibody [EPR3189(2)] was shown to specifically react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type and Laminin beta 1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108536 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (<a href='/en-us/products/primary-antibodies/laminin-beta-1-antibody-epr31892-ab108536'>ab108536</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 1:

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (ab257499)

Lane 2:

LAMB1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lamb1-laminin-beta-1-knockout-hela-cell-line-ab265414'>ab265414</a>)

Predicted band size: 198 kDa

Observed band size: 200 kDa

false

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)
  • WB

Lab

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : LAMB1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab109293 observed at 200 kDa. Red - loading control ab7291 observed at 50 kDa.

ab109293 Anti-Laminin beta 1 antibody [EPR3189(2)] was shown to specifically react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type and Laminin beta 1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109293 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] (<a href='/en-us/products/primary-antibodies/laminin-beta-1-antibody-epr31902-ab109293'>ab109293</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LAMB1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lamb1-laminin-beta-1-knockout-hela-cell-line-ab265414'>ab265414</a>)

Predicted band size: 198 kDa

Observed band size: 200 kDa

false

Sanger Sequencing - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)
  • Sanger seq

Unknown

Sanger Sequencing - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499)

Homozygous : Insertion of the selection cassette in exon3

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
LAMB1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 1 also known as LAMB1 is a subunit of the laminin molecule an essential component of the extracellular matrix. This protein has an approximate molecular weight of 200 kDa. Laminin beta 1 is one of the three chains that form the laminin heterotrimer. It is predominantly expressed in basement membranes which provide structural support and segregate tissues. The laminin 1 (laminin-111) complex including the LAMB1 chain contributes to cell adhesion differentiation migration and plays a role in tissue development and repair.
Biological function summary

This component of the laminin molecule plays a critical role in maintaining the integrity of tissues by organizing cell architecture and mediating cellular signals. Laminin beta 1 is part of a larger laminin protein complex that interacts with a variety of cell surface receptors including integrins and dystroglycan contributing to cell communication and signal transduction. These interactions guide cell orientation and regulate vital processes such as cell movement and growth.

Pathways

Laminin beta 1 participates in the integrin signaling pathway and the PI3K-AKT signaling pathway. It interacts closely with integrin proteins such as integrin alpha-6 and integrin beta-4 during these processes. In integrin signaling the laminin molecule facilitates the binding between cells and their surrounding extracellular matrix. This connection influences cellular responses including survival and motility which are necessary for normal development and wound healing processes.

Alterations in laminin beta 1 expression are linked to conditions like muscular dystrophy and Pierson syndrome. In muscular dystrophy the dysfunction of laminin beta 1 interacts with proteins such as dystrophin in muscle cells contributing to compromised structural integrity and muscle wasting. Similarly in Pierson syndrome abnormalities in laminin beta 1 are associated with a rare genetic disorder that affects the kidneys and eyes where it implicates the protein nephrin in the glomerular basement membrane leading to serious renal dysfunction.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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