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LAMB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

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Images

Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499), expandable thumbnail
  • Western blot - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499), expandable thumbnail
  • Sanger Sequencing - Human LAMB1 (Laminin beta 1) knockout HeLa cell lysate (AB257499), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

Alternative names

CLM, Cutis laxa with marfanoid phenotype, LAMB1_HUMAN, LIS5, Laminin B1, Laminin B1 chain, Laminin beta 1 chain, Laminin beta 1 chain precursor, Laminin beta1, Laminin subunit beta-1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

What's included?

1 Kit
Components
Human LAMB1 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

LAMB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
LAMB1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Laminin beta 1 also known as LAMB1 is a subunit of the laminin molecule an essential component of the extracellular matrix. This protein has an approximate molecular weight of 200 kDa. Laminin beta 1 is one of the three chains that form the laminin heterotrimer. It is predominantly expressed in basement membranes which provide structural support and segregate tissues. The laminin 1 (laminin-111) complex including the LAMB1 chain contributes to cell adhesion differentiation migration and plays a role in tissue development and repair.

Biological function summary

This component of the laminin molecule plays a critical role in maintaining the integrity of tissues by organizing cell architecture and mediating cellular signals. Laminin beta 1 is part of a larger laminin protein complex that interacts with a variety of cell surface receptors including integrins and dystroglycan contributing to cell communication and signal transduction. These interactions guide cell orientation and regulate vital processes such as cell movement and growth.

Pathways

Laminin beta 1 participates in the integrin signaling pathway and the PI3K-AKT signaling pathway. It interacts closely with integrin proteins such as integrin alpha-6 and integrin beta-4 during these processes. In integrin signaling the laminin molecule facilitates the binding between cells and their surrounding extracellular matrix. This connection influences cellular responses including survival and motility which are necessary for normal development and wound healing processes.

Associated diseases and disorders

Alterations in laminin beta 1 expression are linked to conditions like muscular dystrophy and Pierson syndrome. In muscular dystrophy the dysfunction of laminin beta 1 interacts with proteins such as dystrophin in muscle cells contributing to compromised structural integrity and muscle wasting. Similarly in Pierson syndrome abnormalities in laminin beta 1 are associated with a rare genetic disorder that affects the kidneys and eyes where it implicates the protein nephrin in the glomerular basement membrane leading to serious renal dysfunction.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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