Human LAMP3 knockout A549 cell lysate
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LAMP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon2.
View Alternative Names
CD208 antigen, DC LAMP, DC-lysosome-associated membrane glycoprotein, LAMP, LAMP3_HUMAN, Lysosomal-associated membrane protein 3, Lysosome-associated membrane glycoprotein 3, Protein TSC403, TSC403
- Sanger seq
Unknown
Sanger Sequencing - Human LAMP3 knockout A549 cell lysate (AB258492)
Homozygous : 8 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD208 participates in the regulation of the immune response by facilitating antigen processing and presentation. This protein is a part of the lysosomal-associated membrane protein family which contributes to the recycling of MHC class II molecules. Through its involvement in the endocytic pathway CD208 aids in presenting antigens to T lymphocytes an important step in triggering adaptive immunity. The protein colocalizes with MHC class II which is essential for effective antigen presentation.
Pathways
CD208 has a significant role in the endosomal-lysosomal pathway where it interacts with other proteins involved in antigen processing. It also engages in the intracellular trafficking pathway that affects the presentation of peptides on MHC class II molecules. The interaction with proteins such as HLA-DR is vital for efficient antigen presentation to helper T cells. CD208 supports the proper delivery of peptide-loaded MHC molecules to the cell surface a necessity for a functional adaptive immune response.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB271053
Anti-CD208 antibody [EPR24265-8]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] (AB271053)](https://content.abcam.com/products/images/cd208-antibody-epr24265-8-ab271053--multiplex-immunohistochemistry-img427883.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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