Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell lysate
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LAMTOR3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 58 bp deletion in exon5.
View Alternative Names
LAMTOR3, LTOR3_HUMAN, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, MEK partner 1, MEK-binding partner 1, Mitogen-activated protein kinase kinase 1-interacting protein 1, Mitogen-activated protein kinase scaffold protein 1, Mp1, Ragulator complex protein LAMTOR3
- WB
Lab
Western blot - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell lysate (AB258021)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : LAMTOR3 knockout HeLa cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab32134 observed at 14 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32134 Anti-MAP2K1IP1 antibody [Y130] was shown to specifically react with MAP2K1IP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265624 (knockout cell lysate ab258021) was used. Wild-type and MAP2K1IP1 knockout samples were subjected to SDS-PAGE. ab32134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 50000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MAP2K1IP1 antibody [Y130] (<a href='/en-us/products/primary-antibodies/map2k1ip1-antibody-y130-ab32134'>ab32134</a>) at 1/50000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MAP2K1IP1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lamtor3-map2k1ip1-knockout-hela-cell-line-ab265624'>ab265624</a>)
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell lysate (AB258021)
Allele-1 : 58 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human LAMTOR3 (MAP2K1IP1) knockout HeLa cell lysate (AB258021)
Allele-2 : 1 bp insertion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (AB32134)](https://content.abcam.com/products/images/map2k1ip1-antibody-y130-ab32134--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44673.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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