Human LAMTOR4 knockout HEK-293T cell lysate
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LAMTOR4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 2.
View Alternative Names
AV006840, C7orf59, CG059_HUMAN, Chromosome 7 open reading frame 59, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4, Ragulator complex protein LAMTOR4, UPF0539 protein C7orf59
- WB
Lab
Western blot - Human LAMTOR4 knockout HEK-293T cell lysate (AB258939)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : LAMTOR4 knockout HEK-293T cell lysate 20 μg
Lane 3 : MCF7 cell lysate 20 μg
Lane 4 : U-2 OS cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab229738 observed at 11 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab229738 was shown to react with LAMTOR4 in wild-type HEK-293T cells in Western blot with loss of signal observed in LAMTOR4 knockout cell line ab266225 (LAMTOR4 knockout cell lysate ab258939). Wild-type HEK-293T and LAMTOR4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab229738 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Anti-LAMTOR4 antibody (<a href='/en-us/products/unavailable/lamtor4-antibody-ab229738'>ab229738</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
LAMTOR4 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human LAMTOR4 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lamtor4-knockout-hek-293t-cell-line-ab266225'>ab266225</a>)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human LAMTOR4 knockout HEK-293T cell lysate (AB258939)
Homozygous : 4 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a role in nutrient sensing and cellular growth regulation. LAMTOR4 is part of the Ragulator complex a scaffolding complex essential for the activation of mTORC1 a central regulator of cell growth and metabolism. LAMTOR4 contributes to the recruitment and activation of mTORC1 at the lysosomal surface which is important for the integration of nutrient and energetic signals.
Pathways
LAMTOR4 is involved in the mTOR signaling pathway closely related to cellular metabolism and growth. This protein interacts with other members of the Ragulator complex like LAMTOR1 and LAMTOR2 all connecting in the process of mTORC1 activation. Through the mTOR pathway LAMTOR4 modulates autophagy and anabolic processes therefore maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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