Human LARP4B knockout HeLa cell lysate
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LARP4B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 149 bp insertion in exon2.
View Alternative Names
A630096F19, AI256361, D13Wsu64e, KIAA0217, LAR4B_HUMAN, LARP5, La ribonucleoprotein domain family member 4B, La ribonucleoprotein domain family member 5, La-related protein 4B, La-related protein 5, MGC78318
- Sanger seq
Unknown
Sanger Sequencing - Human LARP4B knockout HeLa cell lysate (AB258941)
Homozygous : 149 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LARP4B plays a role in regulating mRNA stability and translation. It often cooperates with mRNA decay and translation initiation factors. LARP4B does not form a stable complex but associates transiently with other RNA-binding proteins to perform its function. Its ability to bind mRNA suggests involvement in gene expression post-transcriptionally impacting various cellular processes.
Pathways
LARP4B is implicated in the mTOR signaling pathway and the RNA processing pathway. The mTOR pathway is essential for cell growth and proliferation linking nutrient availability to these processes. LARP4B works in tandem with other proteins like LARP1 and EIF4E to modulate mRNA stability indirectly influencing cell growth through translation regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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