Human LDHB (Lactate Dehydrogenase B) knockout HEK-293T cell lysate
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LDHB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
Epididymis secretory protein Li 281, HEL S 281, L-lactate dehydrogenase B chain, LDH heart subunit, LDH-H, LDHBD, LDHB_HUMAN, Lactate Dehydrogenase B, Lactate dehydrogenase H chain, Renal carcinoma antigen NY-REN-46, TRG-5
- WB
Lab
Western blot - Human LDHB (Lactate Dehydrogenase B) knockout HEK-293T cell lysate (AB263761)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : LDHB knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control ab130007 observed at 124 kDa.
ab53292 Recombinant Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] was shown to specifically react with LDHB in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type and LDHB knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab53292 and Anti-Vinculin antibody [VIN-54] were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (<a href='/en-us/products/primary-antibodies/lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-ab53292'>ab53292</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
LDHB knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human LDHB (Lactate Dehydrogenase B) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ldhb-lactate-dehydrogenase-b-knockout-hek-293t-cell-line-ab255403'>ab255403</a>)
Predicted band size: 37 kDa
Observed band size: 37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human LDHB (Lactate Dehydrogenase B) knockout HEK-293T cell lysate (AB263761)
Homozygous : 1 bp insertion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.
Pathways
Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB52488
Anti-Lactate Dehydrogenase antibody [EP1566Y]
20ul selling size|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (AB52488)](https://content.abcam.com/products/images/lactate-dehydrogenase-antibody-ep1566y-ab52488--immunocytochemistry-immunofluorescence-img120364.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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