Human LGALS1 (Galectin 1) knockout HEK-293T cell lysate
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LGALS1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 171 bp deletion in exon 2.
View Alternative Names
14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding lectin, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GALBP, Gal-1, Galaptin, Galectin, Galectin-1, HBL, HLBP14, HPL, L-14.5, L14, LEG1_HUMAN, LGALS 1, Lactose-binding lectin 1, Lect14, Lectin galactoside-binding soluble 1, Lgals1 lectin galactose binding soluble 1, MAPK activating protein MP12, Putative MAPK activating protein MP12, Putative MAPK-activating protein PM12, S-Lac lectin 1, beta galactoside binding protein
- WB
Lab
Western blot - Human LGALS1 (Galectin 1) knockout HEK-293T cell lysate (AB256974)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : LGALS1 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control ab8245 observed at 37 kDa.
ab138513 Anti-Galectin 1 antibody [EPR3206(2)] was shown to specifically react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266850 (knockout cell lysate ab256974) was used. Wild-type and Galectin 1 knockout samples were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (<a href='/en-us/products/primary-antibodies/galectin-1-antibody-epr32062-ab138513'>ab138513</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
LGALS1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human LGALS1 (Galectin 1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lgals1-galectin-1-knockout-hek-293t-cell-line-ab266850'>ab266850</a>)
Predicted band size: 15 kDa
Observed band size: 15 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human LGALS1 (Galectin 1) knockout HEK-293T cell lysate (AB256974)
Homozygous : 171 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB138513
Anti-Galectin 1 antibody [EPR3206(2)]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513)](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-ab138513--western-blot-img4866.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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