Human LIN7C knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human LIN7C knockout HeLa cell lysate (AB258942)
Allele-1 : 26 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LIN7C knockout HeLa cell lysate (AB258942)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LIN7C knockout HeLa cell lysate (AB258942)
Allele-3 : 7 bp insertion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LIN7C contributes significantly to cellular signaling and organization. It operates as part of a multiprotein complex alongside DLG1 and CASK interacting with several other proteins to facilitate cell adhesion and synaptic transmission. This assembly orchestrates the anchoring of membrane proteins influencing the spatial distribution of signaling receptors. LIN7C's role ensures that proteins reach and maintain their proper membrane location important for successful synaptic and epithelial cell functioning.
Pathways
LIN7C is actively involved in the PI3K/AKT pathway critical for cell survival and proliferation. LIN7C assists in stabilizing interactions with signaling molecules like GRIN2B contributing to synaptic plasticity and memory formation. Additionally LIN7C engages with the Wnt signaling pathway which influences cell development and differentiation through interactions with proteins such as LRP6. This interaction emphasizes LIN7C's importance in processes that guide cellular growth and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com