JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258943

Human LIX1L knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

LIX1L KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.
2 Images
Sanger Sequencing - Human LIX1L knockout HeLa cell lysate (AB258943)
  • Sanger seq

Unknown

Sanger Sequencing - Human LIX1L knockout HeLa cell lysate (AB258943)

Allele-2 : 1 bp insertion in exon 1

Sanger Sequencing - Human LIX1L knockout HeLa cell lysate (AB258943)
  • Sanger seq

Unknown

Sanger Sequencing - Human LIX1L knockout HeLa cell lysate (AB258943)

Allele-1 : 5 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Cell Lines & Lysates

AB265950

Human LIX1L knockout HeLa cell line

Sanger Sequencing - Human LIX1L knockout HeLa cell line (AB265950)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258943-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human LIX1L knockout HeLa cell lysate", "number":"AB258943-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258943-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
LIX1L
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIX1L also known as Lim Homeobox Protein 1-Like is a protein encoded by the LIX1L gene with a mass of approximately 28.8 kDa. This protein is expressed in various tissues including the brain and testis indicating its role in multiple physiological contexts. LIX1L's structure suggests it could interact with DNA or proteins but its precise molecular mechanisms remain an area for further study.
Biological function summary

Lim Homeobox Protein 1-Like participates in processes linked to development and differentiation. It has been implicated in nervous system development possibly through interactions with transcriptional regulators. LIX1L may form part of larger protein complexes aligning with its expression in neural and reproductive tissues. Its presence in these tissues indicates roles in establishing cellular identity and tissue architecture.

Pathways

Lim Homeobox Protein 1-Like plays functions in developmental signaling pathways such as Wnt and Hedgehog. These pathways are critical for cell fate determination and tissue patterning. LIX1L might interact with proteins like beta-catenin in the Wnt signaling cascade and Gli in the Hedgehog pathway influencing their pathways and cellular outcomes.

LIX1L associates with conditions affecting the nervous system and reproductive organs. Neurological disorders like certain congenital brain malformations link with abnormal expression of LIX1L. Additionally reproductive issues possibly including infertility might arise from LIX1L's dysregulation. Studies suggest these associations through LIX1L's interactions with proteins such as GLI3 in the context of Hedgehog pathway dysregulation.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com