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AB256979

Human LMNA (Lamin A) knockout HeLa cell lysate

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LMNA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon4 and 1 bp deletion in exon4 and 22 bp deletion in exon4.

View Alternative Names

70 kDa lamin, CDDC, EMD2, FPL, FPLD, HGPS, IDC, LAMIN A, LAMIN C, LDP1, LFP, LMN 1, LMN C, LMNA_HUMAN, Lamin-A/C, PRO1, Renal carcinoma antigen NY-REN-32

5 Images
Western blot - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)
  • WB

Supplier Data

Western blot - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : LMNA knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab232730 observed at 70,75 kDa. Red - loading control ab181602 observed at 37 kDa.

ab232730 Anti-Lamin A + C antibody [WL4G10] was shown to specifically react with Lamin A + C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261787 (knockout cell lysate ab256979) was used. Wild-type and Lamin A + C knockout samples were subjected to SDS-PAGE. ab232730 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [WL4G10] (<a href='/en-us/products/primary-antibodies/lamin-a-lamin-c-antibody-wl4g10-ab232730'>ab232730</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Lamin A + C knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LMNA (Lamin A) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lmna-lamin-a-knockout-hela-cell-line-ab261787'>ab261787</a>)

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 70 kDa,75 kDa

false

Western blot - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)
  • WB

Lab

Western blot - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : LMNA knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab238303 observed at 74 kDa. Red - loading control ab181602 observed at 37 kDa.

ab238303 Anti-Lamin A + C antibody [4C11] was shown to specifically react with Lamin A + C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261787 (knockout cell lysate ab256979) was used. Wild-type and Lamin A + C knockout samples were subjected to SDS-PAGE. ab238303 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [4C11] (<a href='/en-us/products/primary-antibodies/lamin-a-lamin-c-antibody-4c11-ab238303'>ab238303</a>) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Lamin A + C knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LMNA (Lamin A) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lmna-lamin-a-knockout-hela-cell-line-ab261787'>ab261787</a>)

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 74 kDa

false

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)
  • Sanger seq

Unknown

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)

Allele-3 : 22 bp deletion in exon4

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)
  • Sanger seq

Unknown

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)

Allele-2 : 1 bp deletion in exon4

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)
  • Sanger seq

Unknown

Sanger Sequencing - Human LMNA (Lamin A) knockout HeLa cell lysate (AB256979)

Allele-1 : 19 bp deletion in exon4

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon4 and 1 bp deletion in exon4 and 22 bp deletion in exon4.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256979-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human LMNA knockout HeLa cell lysate", "number":"AB256979-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB256979-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
LMNA
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A also known as LMNA is a type of lamin protein integral to the nuclear architecture. It has a molecular weight of approximately 70 kDa. This protein localizes mainly to the nuclear lamina a dense fibrillar network inside the inner nuclear membrane found across various cell types. Lamin A serves as a structural scaffold mediating nuclear stability and rigidity. It also plays a role in chromatin organization supporting overall nuclear functions.
Biological function summary

The lamin molecule interacts with several nuclear components forming part of the nuclear lamina complex. It anchors chromatin material and regulates DNA replication and repair. Lamin A protein also modulates gene expression through interactions with transcription factors. Beyond structural support it influences cell cycle progression and differentiation impacting cellular mechanotransduction and signaling processes.

Pathways

Lamin A performs critical functions within the cell cycle and apoptotic pathways. Its interactions with the retinoblastoma protein (pRB) and other cyclin-dependent kinases control cell cycle checkpoints and progression. Moreover lamin A connects with proteins involved in signaling pathways like MAPK which relate to stress responses and cellular growth. These interactions highlight its dynamic involvement in maintaining cell health and proliferation.

Mutations in the lamin A gene associate closely with Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. In these conditions altered lamin A protein affects nuclear shape and chromatin layout disrupting transcriptional regulation. The mutated lamin A protein interacts differently with binding partners like emerin contributing to muscle adipose tissue and multi-system abnormalities. This highlights the critical role lamin A plays in maintaining normal cellular function across life stages.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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