Skip to main content

LOXL2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.

Be the first to review this product! Submit a review

Images

Western blot - Human LOXL2 knockout HeLa cell lysate (AB257168), expandable thumbnail
  • Western blot - Human LOXL2 knockout HeLa cell lysate (AB257168), expandable thumbnail
  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (AB257168), expandable thumbnail
  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (AB257168), expandable thumbnail
  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (AB257168), expandable thumbnail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.

Alternative names

What's included?

1 Kit
Components
Human LOXL2 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

LOXL2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.

Key facts

Cell type

HeLa

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.

Disease

Adenocarcinoma

Concentration
Loading...

Properties

Gene name

LOXL2

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Lane 1: Wild-type HeLa cell lysate (20 μg)

    Lane 2: LOXL2 knockout HeLa cell lysate (20 μg)

    Lane 3: HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate (20 μg)

    Lane 4: Untreated HeLa cell lysate (20 μg)

    Lanes 1-4: Merged signal (red and green). Green - Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 observed at 105 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human LOXL2 knockout HeLa cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810) at 1/500 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: LOXL2 knockout HeLa cell lysate at 20 µg

    Lane 4: Untreated HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 87 kDa

    Observed band size: 105 kDa

  • Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS

    Negative control: MCF7 (PMID: 19330836).

    Lysates at 20 µg per lane.

    The samples were run on a Bis-Tris gel under reducing conditions.

    Western blot: Anti-LOXL2 antibody (Anti-LOXL2 antibody [EPR28298-7] ab314140) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, Anti-LOXL2 antibody [EPR28298-7] ab314140 was shown to bind specifically to LOXL2. Target of interest was observed at 87 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in LOXL2 knockout cell line (lane 2, knockout cell line Human LOXL2 knockout HeLa cell line ab261804 / knockout cell lysate ab257168). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane.  Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C.  Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

    All lanes: Western blot - Anti-LOXL2 antibody [EPR28298-7] (Anti-LOXL2 antibody [EPR28298-7] ab314140) at 1/1000 dilution

    Lane 1: Wild-type HeLa whole cell lysate at 20 µg

    Lane 2: Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168) at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 87 kDa

  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Allele-4:

  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Allele-3:

  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Allele-1: 1 bp insertion in exon 4

  • Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168), expandable thumbnail

    Sanger Sequencing - Human LOXL2 knockout HeLa cell lysate (ab257168)

    Allele-2:

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com