LOXL2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.
HeLa
Human
Cervix
Sanger Sequencing, Western blot
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.
LOR 2, LOXL2_HUMAN, Lysyl oxidase homolog 2, Lysyl oxidase like 2, Lysyl oxidase related 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, WS9 14
LOXL2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.
HeLa
Human
Cervix
Sanger Sequencing, Western blot
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4.
Adenocarcinoma
LOXL2
Knockout
CRISPR technology
Sanger Sequencing, Western blot
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
EU: 2 US: 2
Adherent
Female
Ambient - Can Ship with Ice
-20°C
-20°C
Knockout cell lysate achieved by CRISPR/Cas9.
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Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
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Lane 1: Wild-type HeLa cell lysate (20 μg)
Lane 2: LOXL2 knockout HeLa cell lysate (20 μg)
Lane 3: HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate (20 μg)
Lane 4: Untreated HeLa cell lysate (20 μg)
Lanes 1-4: Merged signal (red and green). Green - Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 observed at 105 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human LOXL2 knockout HeLa cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (Anti-LOXL2 antibody [EPR12733] - C-terminal ab179810) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: LOXL2 knockout HeLa cell lysate at 20 µg
Lane 4: Untreated HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 105 kDa
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS
Negative control: MCF7 (PMID: 19330836).
Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-LOXL2 antibody (Anti-LOXL2 antibody [EPR28298-7] ab314140) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-LOXL2 antibody [EPR28298-7] ab314140 was shown to bind specifically to LOXL2. Target of interest was observed at 87 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in LOXL2 knockout cell line (lane 2, knockout cell line Human LOXL2 knockout HeLa cell line ab261804 / knockout cell lysate ab257168). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
All lanes: Western blot - Anti-LOXL2 antibody [EPR28298-7] (Anti-LOXL2 antibody [EPR28298-7] ab314140) at 1/1000 dilution
Lane 1: Wild-type HeLa whole cell lysate at 20 µg
Lane 2: Western blot - Human LOXL2 knockout HeLa cell lysate (ab257168) at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 87 kDa
Allele-4:
Allele-3:
Allele-1: 1 bp insertion in exon 4
Allele-2:
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