Human LRP5 knockout HEK-293T cell lysate
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LRP5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.
View Alternative Names
BMND1, EVR1, EVR4, HBM, LR3, LRP5_HUMAN, LRP7, Low density lipoprotein receptor related protein 7, Low-density lipoprotein receptor-related protein 5, OPPG, OPS, OPTA1, Osteoporosis pseudoglioma syndrome, VBCH2
- WB
Lab
Western blot - Human LRP5 knockout HEK-293T cell lysate (AB257202)
Lane 1 : Wild-type HEK293T cell lysate (20 μg)
Lane 2 : LRP5 knockout HEK293T cell lysate (20 μg)
Lane 3 : SW620 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab223203 observed at 180-200 kDa. Red - loading control ab7291 observed at 50 kDa.
ab223203 Anti-LRP5 antibody [EPR22477-218] was shown to specifically react with LRP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266618 (knockout cell lysate ab257202) was used. Wild-type and LRP5 knockout samples were subjected to SDS-PAGE. ab223203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-LRP5 antibody [EPR22477-218] (<a href='/en-us/products/primary-antibodies/lrp5-antibody-epr22477-218-ab223203'>ab223203</a>) at 1/500 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
LRP5 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human LRP5 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lrp5-knockout-hek-293t-cell-line-ab266618'>ab266618</a>)
Lane 3:
SW620 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 179 kDa
Observed band size: 180-200 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human LRP5 knockout HEK-293T cell lysate (AB257202)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human LRP5 knockout HEK-293T cell lysate (AB257202)
Allele-1 : 5 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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