Human LRRC58 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human LRRC58 knockout HeLa cell lysate (AB258500)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LRRC58 knockout HeLa cell lysate (AB258500)
Allele-3 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LRRC58 knockout HeLa cell lysate (AB258500)
Allele-1 : 7 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LRRC58 contributes to cellular processes through its involvement in signaling pathways. While detailed functions are under study there is evidence suggesting that it could form part of larger protein complexes influencing downstream molecular signaling. This interaction possibly regulates cellular growth and differentiation important for maintaining healthy cellular environments.
Pathways
Scientists have linked LRRC58 to the MAPK/ERK signaling pathway which is important in regulating cell division and differentiation. It may interact with proteins such as MEK1/2 and ERK1/2 in this context. Another possible relationship exists with the PI3K/AKT pathway which indicates a role in cell survival and metabolism regulation. The exact mechanism of LRRC58's involvement in these pathways is an area of active research.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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