Human LRRC59 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human LRRC59 knockout HEK-293T cell lysate (AB263247)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LRRC59 knockout HEK-293T cell lysate (AB263247)
Allele-1 : Insertion of the selection cassette in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LRRC59 is involved in nuclear import pathways and acts as an anchor for certain proteins that need to be translocated into the nucleus. It interacts with the importin-family proteins to facilitate the transport of proteins with nuclear localization signals. LRRC59 works as a part of a larger protein complex which includes importin and nucleoporins to control transport into the nucleus. This positioning suggests that LRRC59 has an active role in regulating gene expression by controlling the nuclear entry of transcription factors and other nuclear proteins.
Pathways
LRRC59 plays a part in cellular transport and signaling pathways. It integrates into the nuclear transport pathway directly influencing gene expression by ensuring the proper nuclear import of signaling molecules. In particular it influences the well-known MAPK signaling pathway through interactions with other proteins like importin-β. These associations emphasize LRRC59’s role in maintaining cellular homeostasis and its potential impact on several signaling cascades.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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