Human LRWD1 knockout HeLa cell lysate
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LRWD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
1200011O22Rik, AU042569, AW548074, DKFZp434K1815, LRWD1_HUMAN, Leucine rich repeats and WD repeat domain containing 1, Leucine-rich repeat and WD repeat-containing protein 1, ORC-associated protein, ORCA, Origin recognition complex-associated protein
- Sanger seq
Unknown
Sanger Sequencing - Human LRWD1 knockout HeLa cell lysate (AB258502)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human LRWD1 knockout HeLa cell lysate (AB258502)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LRWD1 supports the regulation of DNA replication and centrosome cycle. It is an integral part of the DNA replication licensing pathway ensuring proper DNA synthesis during the cell cycle. By participating in this complex LRWD1 collaborates with proteins like CDC6 and CDT1 to initiate DNA replication. This protein ensures that cells duplicate their DNA once per cycle maintaining genomic stability.
Pathways
LRWD1 participates in the DNA replication pathway and is also linked to the G2 to M phase transition of the cell cycle. Through the DNA replication pathway LRWD1 works hand-in-hand with other factors such as ORC1 and MCM proteins which coordinate the licensing of replication origins. Its presence in these pathways demonstrates its pivotal role in maintaining normal cell cycle progression and genomic integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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