Human LTB4R2 (Leukotriene B4 Receptor 2) knockout HeLa cell lysate
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LTB4R2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 22 bp deletion in exon2.
View Alternative Names
BLT2R, JULF2, LT4R2_HUMAN, LTB4 receptor JULF2, LTB4-R 2, Leukotriene B4 receptor 2, Leukotriene B4 receptor BLT2, Seven transmembrane receptor BLTR2
- Sanger seq
Unknown
Sanger Sequencing - Human LTB4R2 (Leukotriene B4 Receptor 2) knockout HeLa cell lysate (AB258035)
Homozygous : 22 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The receptor BLT2 significantly contributes to the regulation of inflammatory responses. This receptor does not form part of a functional complex but operates independently to mediate chemotaxis and aggregation of immune cells like neutrophils. By interacting with leukotriene B4 BLT2 facilitates diverse immune responses including leukocyte recruitment and activation. Such activities underline its importance in acute inflammatory reactions and pathogen defense mechanisms.
Pathways
BLT2 influences and integrates into key cellular signaling routes such as the MAPK/ERK and PI3K/Akt pathways. These pathways affect cellular processes like proliferation survival and differentiation. BLT2's interaction with proteins involved in these pathways like ERK (Extracellular Signal-Regulated Kinases) or Akt (protein kinase B) demonstrate its role in amplifying and regulating immune responses during prolonged inflammation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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