Human LUC7L2 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human LUC7L2 knockout HEK-293T cell lysate (AB258036)
Homozygous : 1 bp insertion in exon 2
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Cell Lines & Lysates
AB258523
Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LUC7L2 is an important factor in the regulation of gene expression. It is part of the spliceosome complex which is responsible for the precisely regulated removal of introns from pre-mRNA. This activity impacts the generation of mature mRNA and consequently protein synthesis. LUC7L2 interacts with other components of the spliceosome like the U1 snRNP which ensures accurate and efficient splicing. Through these interactions LUC7L2 helps maintain the fidelity of the splicing process affecting cell function and health.
Pathways
LUC7L2 participates in the mRNA splicing pathway also known as the spliceosome cycle. This protein is involved in both the recognition of splice sites and the catalytic steps of splicing. LUC7L2's activity influences alternative splicing patterns which diversifies the proteome. It interacts with other proteins like the SR proteins which modulate splicing decisions. Its role in the spliceosome cycle highlights its contribution to gene expression regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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