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AB257509

Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell lysate

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LYPLA1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon1.
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Western blot - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell lysate (AB257509)
  • WB

Lab

Western blot - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell lysate (AB257509)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : LYPLA1 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

ab91606 Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] was shown to specifically react with Lysophospholipase 1/LPL-I in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266195 (knockout cell lysate ab257509) was used. Wild-type and Lysophospholipase 1/LPL-I knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab91606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (<a href='/en-us/products/primary-antibodies/lysophospholipase-1-lpl-i-antibody-epr3667-ab91606'>ab91606</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

LYPLA1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lypla1-lysophospholipase-1-lpl-i-knockout-hek-293t-cell-line-ab266195'>ab266195</a>)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Sanger Sequencing - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell lysate (AB257509)
  • Sanger seq

Unknown

Sanger Sequencing - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell lysate (AB257509)

Homozygous : 2 bp deletion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon1.

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Complementary Products

Primary Antibodies

AB91606

Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

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Cell Lines & Lysates

AB266195

Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell line

Advanced Validation

Western blot - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell line (AB266195)

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Proteins & Peptides

AB151829

Recombinant Human Lysophospholipase 1/LPL-I protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
LYPLA1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysophospholipase 1 also known as LPL-I is an enzyme involved in lipid metabolism. It catalyzes the hydrolysis of lysophospholipids into fatty acids and glycerophospholipids. The molecular mass of Lysophospholipase 1 is approximately 25 kDa. This protein is expressed in various tissues including heart liver and brain. Alternate names for this enzyme include APEH and acylaminoacyl-peptidase reflecting its ability to hydrolyze peptides and acyl groups.
Biological function summary

Lysophospholipase 1 plays a role in maintaining lipid homeostasis. It does not function as part of a larger protein complex acting independently in the regulation of lipid turnover. By breaking down lysophospholipids the enzyme helps balance the cellular lipid composition ensuring proper membrane integrity and cellular signaling. This regulation is critical in tissues that have high turnover of lipids like the brain and liver.

Pathways

Lysophospholipase 1 participates in lipid metabolism and glycerophospholipid biosynthesis pathways. The enzyme helps process intermediates in these pathways facilitating energy production and phospholipid remodeling. In terms of related proteins LPL-I functions in coordination with phospholipase A2 in lipid degradation and this relationship impacts the synthesis of bioactive lipid mediators.

Lysophospholipase 1 has been linked to metabolic disorders and neurological diseases. Altered activity of this enzyme has correlations with conditions such as metabolic syndrome where lipid imbalances occur. Moreover its dysregulation can influence neurological disorders like Alzheimer's Disease due to its impact on lipid signaling. In these contexts LPL-I interacts functionally with other enzymes like phospholipase A2 which also plays a role in lipid signaling and homeostasis.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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