Human LYZ (Lysozyme) knockout THP-1 cell lysate
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LYZ KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 20 bp deletion Frameshift: 94.11%.
View Alternative Names
1 4 beta N acetylmuramidase C, 4-beta-N-acetylmuramidase C, EC 3.2.1.17, LYSC_HUMAN, LYZ, LZM, Lysosyme, Lysozyme (renal amyloidosis), Lysozyme C, Lysozyme C precursor, Renal amyloidosis
- WB
Lab
Western blot - Human LYZ (Lysozyme) knockout THP-1 cell lysate (AB270711)
Lane 1 : Wild-type THP-1 (Human monocytic leukemia cell line) whole cell lysate 20 ug
Lane 2 : LYZ knockout THP-1 (Human monocytic leukemia cell line) whole cell lysate 20 ug
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate 20 ug
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab108508 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab108508 was shown to react with Lysozyme in wild-type THP-1 cells in Western blot with loss of signal observed in LYZ knockout cell line ab269595 (knockout cell lysate ab270711). Wild-type THP-1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108508 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (<a href='/en-us/products/primary-antibodies/lysozyme-antibody-epr29942-ab108508'>ab108508</a>) at 1/10000 dilution
Lane 1:
Wild-type THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg
Lane 2:
LYZ knockout THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg
Lane 2:
Western blot - Human LYZ (Lysozyme) knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-lyz-lysozyme-knockout-thp-1-cell-line-ab269595'>ab269595</a>)
Lane 3:
Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 4:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Predicted band size: 16 kDa,68 kDa,71 kDa
Observed band size: 16 kDa,58 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human LYZ (Lysozyme) knockout THP-1 cell lysate (AB270711)
Knockout achieved by CRISPR/Cas9; X = 20 bp deletion; Frameshift : 94.11%
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.
Pathways
Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Complementary Products
Cell Lines & Lysates
AB273760
Human CCL3 (Macrophage Inflammatory Protein 1) knockout THP-1 cell line
- 0
- 0
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508)](https://content.abcam.com/products/images/lysozyme-antibody-epr29942-ab108508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113098.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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