Human MAGEA6 knockout HeLa cell lysate
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MAGEA6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and Insertion of the selection cassette in exon3.
View Alternative Names
CT1.6, Cancer/testis antigen 1.6, Cancer/testis antigen family 1, member 6, MAGA6_HUMAN, MAGE 3b, MAGE-6 antigen, MAGE3B antigen, MAGE6, Melanoma antigen family A 6, Melanoma-associated antigen 6
- Sanger seq
Unknown
Sanger Sequencing - Human MAGEA6 knockout HeLa cell lysate (AB258951)
Allele-1 : 1 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human MAGEA6 knockout HeLa cell lysate (AB258951)
Allele-2 : Insertion of the selection cassette in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MAGEA6 is involved in cellular processes relating to gene regulation and apoptosis. It interacts with other MAGE proteins and is often part of a larger multiprotein complex which influences gene transcription. The protein plays a role in cell survival and proliferation particularly in tumorous cells where it can aid in escape from immune surveillance.
Pathways
MAGEA6 participates in critical signaling pathways including the p53 pathway. This pathway is significant in regulating cell cycle arrest DNA repair and apoptosis. MAGEA6 can interact with proteins like p53 and MDM2 potentially modulating the tumor suppressor functions of p53. This interaction highlights its role in tumor pathogenesis as it can affect cell cycle regulation and apoptosis resistance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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