Human MALT1 (MLT) knockout HeLa cell lysate
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MALT1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
View Alternative Names
Caspase like protein, DKFZp434L132, IMD12, MALT associated translocation, MALT lymphoma-associated translocation, MALT1 paracaspase, MALT1_HUMAN, MLT 1, Mucosa associated lymphoid tissue lymphoma translocation gene 1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, PCASP1, Paracaspase, Paracaspase-1
- WB
Lab
Western blot - Human MALT1 (MLT) knockout HeLa cell lysate (AB257149)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : MALT1 knockout HeLa cell lysate (20 μg)
Lane 3 : Ramos cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab33921 observed at 92 kDa. Red - loading control ab7291 observed at 50 kDa.
ab33921 Anti-MALT1/MLT antibody [EP603Y] was shown to specifically react with MALT1/MLT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264930 (knockout cell lysate ab257149) was used. Wild-type and MALT1/MLT knockout samples were subjected to SDS-PAGE. ab33921 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MALT1/MLT antibody [EP603Y] (<a href='/en-us/products/primary-antibodies/malt1-mlt-antibody-ep603y-ab33921'>ab33921</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MALT1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human MALT1 (MLT) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-malt1-mlt-knockout-hela-cell-line-ab264930'>ab264930</a>)
Lane 3:
Ramos cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MALT1 (MLT) knockout HeLa cell lysate (AB257149)
Homozygous : Insertion of the selection cassette in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (AB33921)](https://content.abcam.com/products/images/malt1-mlt-antibody-ep603y-ab33921--flow-cytometry-intracellular-img39268.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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