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AB258952

Human MAN1C1 (HMIC) knockout HeLa cell lysate

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MAN1C1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 28 bp deletion in exon3.
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Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)

Allele-1 : 28 bp deletion in exon3

Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)

Allele-2 : 1 bp deletion in exon3

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 28 bp deletion in exon3.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265977

Human MAN1C1 (HMIC) knockout HeLa cell line

Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell line (AB265977)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MAN1C1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMIC also known as Histone Modifier Inhibitor Complex is a protein complex involved in chromatin modification. It plays an active role in the regulation of gene expression by altering chromatin structure. The complex has a mass of approximately 120 kDa and is found in the nucleus of the cell. Researchers have observed its expression in various tissues including muscle liver and brain indicating its widespread role in cellular processes.
Biological function summary

HMIC influences transcription regulation by participating in the modification of histone tails. It forms part of a larger multi-protein assembly that targets specific lysine residues on histones. This modification alters the chromatin's accessibility to transcription machinery impacting gene expression levels. By modulating histone acetylation and methylation states HMIC links closely with cellular processes like DNA replication and repair.

Pathways

HMIC functions within the chromatin remodeling pathways especially affecting the gene transcription pathway. It involves interactions with proteins such as transcription factors and other chromatin remodelers like Polycomb group proteins. These interactions enable the precise regulation of genes necessary for cell differentiation and development as well as in response to environmental signals.

Abnormal activity of HMIC associates with oncogenesis and neurodegenerative diseases. Its dysregulation can lead to improper gene expression patterns that contribute to cancerous growths or neurodegenerative conditions like Alzheimer's disease. The complex interacts with proteins such as p53 linking its misregulation to tumor development and progression. Similarly altered HMIC activity influences proteins responsible for neuronal survival and function highlighting its relevance in neurobiology.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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