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AB257516

Human MAP2K7 (MKK7) knockout A549 cell lysate

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MAP2K7 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.
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Sanger Sequencing - Human MAP2K7 (MKK7) knockout A549 cell lysate (AB257516)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAP2K7 (MKK7) knockout A549 cell lysate (AB257516)

Homozygous : 1 bp insertion in exon6

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB52618

Anti-MKK7 antibody [EP1455Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MKK7 antibody [EP1455Y] (AB52618)

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Cell Lines & Lysates

AB266914

Human MAP2K7 (MKK7) knockout A549 cell line

Advanced Validation

Sanger Sequencing - Human MAP2K7 (MKK7) knockout A549 cell line (AB266914)

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  • 0
Primary Antibodies

AB76125

Anti-JNK2 antibody [EP1595Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK2 antibody [EP1595Y] (AB76125)

  • 5
  • 1
Primary Antibodies

AB179461

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

  • 4
  • 7
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MAP2K7
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MKK7 also known as MAP2K7 or Mitogen-activated protein kinase kinase 7 is a dual-specificity kinase involved in the phosphorylation of target proteins. It functions as a critical member of the MAP2K family with a molecular mass of approximately 44 kDa. MKK7 is widely expressed in various tissues including the brain and immune cells which indicates its diverse functional roles across different biological systems.
Biological function summary

MKK7 plays an essential role in the activation of the JNK (c-Jun N-terminal kinases) signaling pathway by phosphorylating and activating JNK. It operates as part of a signaling complex that responds to stress stimuli. This kinase is involved in numerous cellular processes such as apoptosis differentiation and inflammatory responses. The regulation of these processes by MKK7 impacts many aspects of cellular homeostasis and response to environmental changes.

Pathways

MKK7 is integrally involved in the stress-activated MAPK pathway and the cellular response to inflammatory cytokines. It closely collaborates with related proteins such as JNK1 JNK2 and JNK3 to mediate its downstream effects. Activation of this pathway leads to the modulation of gene expression that governs cell survival proliferation and other critical cellular functions.

Dysregulation of MKK7 activity connects the protein to disorders like cancer and neurodegenerative diseases. Altered MKK7-JNK signaling contributes to the pathogenesis of certain cancers by affecting cell proliferation and survival mechanisms. In neurodegenerative diseases MKK7-mediated pathways are associated with neuronal stress responses and cell death highlighting its interaction with proteins like Amyloid-beta in Alzheimer's disease. Understanding MKK7's role in these conditions offers potential for therapeutic interventions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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