Human MAP3K10 (MLK2) knockout HEK-293T cell lysate
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MAP3K10 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 8 bp deletion in exon1.
View Alternative Names
M3K10_HUMAN, MAP3K 10, MEKK10, MKN28 derived nonreceptor type serine/threonine kinase, MKN28 kinase, Mitogen-activated protein kinase kinase kinase 10, Mixed lineage kinase 2, Protein kinase MST
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K10 (MLK2) knockout HEK-293T cell lysate (AB258505)
Allele-1 : 8 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K10 (MLK2) knockout HEK-293T cell lysate (AB258505)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase influences cellular dynamics by partaking in the MAP kinase signal transduction cascade and is a component of signaling complexes. MLK2 is important in the regulation of cell proliferation differentiation and apoptosis. It primarily transduces signals originating from cell surface receptors through multi-step phosphorylation events affecting downstream effectors. MLK2 interacts with MAP kinase kinases (MKKs) to activate c-Jun N-terminal kinases (JNKs) and p38 pathways.
Pathways
MLK2 acts within the MAPK signaling and JNK pathways. It regulates responses to environmental stresses and cytokines therefore modulating cell fate. MLK2 has functional associations with proteins like JNKs and MKKs which play leading roles in these pathways. Furthermore it works in conjunction with other MAP3Ks to regulate MAPK signaling cascades tightly.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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