Human MAP3K11 (MLK3) knockout HeLa cell lysate
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MAP3K11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp deletion in exon 1.
View Alternative Names
2610017K16Rik, EC 2.7.11.25, M3K11_HUMAN, MEKK11, MGC17114, Map3k11, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Mixed lineage protein kinase 3, PTK1, Protein tyrosine kinase PTK1, RHOE, SH3 domain containing proline rich kinase, SPRK, Src-homology 3 domain-containing proline-rich kinase
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell lysate (AB257517)
Allele-2 : 1 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell lysate (AB257517)
Allele-1 : 11 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MLK3 plays a critical role in cellular response to stress and inflammation. It acts as part of a signaling complex that regulates the JNK and p38 pathways key mediators in immune responses. These pathways control the production of pro-inflammatory cytokines and the cellular stress response. Through its kinase activity MLK3 supports the activation of transcription factors such as AP-1 which drive gene expression changes needed for its biological functions.
Pathways
MLK3 is intricately involved in the MAPK signaling cascade. It connects with other MAP3Ks like MLK1 and MLK2 contributing to the complexity of MAPK signaling. MLK3’s participation in the JNK and p38 pathways links it to cytokine signaling and cellular stress responses. These pathways are significant for maintaining cellular homeostasis and coordinating responses to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB51068
RabMAb|Recombinant|KO Validated|Trial Size
![Western blot - Anti-MLK3 antibody [EP1460Y] (AB51068)](https://content.abcam.com/products/images/mlk3-antibody-ep1460y-ab51068--western-blot-img58162.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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