Human MAP3K2 (MEKK2) knockout A549 cell lysate
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MAP3K2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 2 bp deletion in exon7.
View Alternative Names
M3K2_HUMAN, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK2b, Map3k2, Mitogen-activated protein kinase kinase kinase 2
- WB
Lab
Western blot - Human MAP3K2 (MEKK2) knockout A549 cell lysate (AB257521)
Lane 1 : Wild-type A549 cell lysate (20µg)
Lane 2 : MAP3K2 knockout A549 cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab33918 observed at 75 kDa. Red - loading control ab8245 observed at 37 kDa.
ab33918 Anti-MEKK2 antibody [EP626Y] was shown to specifically react with MEKK2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267152 (knockout cell lysate ab257521) was used. Wild-type and MEKK2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab33918 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MEKK2 antibody [EP626Y] (<a href='/en-us/products/primary-antibodies/mekk2-antibody-ep626y-ab33918'>ab33918</a>) at 1/10000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
MAP3K2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human MAP3K2 (MEKK2) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-map3k2-mekk2-knockout-a549-cell-line-ab267152'>ab267152</a>)
Predicted band size: 70 kDa
Observed band size: 75 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K2 (MEKK2) knockout A549 cell lysate (AB257521)
Allele-1 : 2 bp deletion in exon7
- Sanger seq
Unknown
Sanger Sequencing - Human MAP3K2 (MEKK2) knockout A549 cell lysate (AB257521)
Allele-2 : 1 bp insertion in exon7
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase serves as a critical mediator in cellular processes like proliferation differentiation and apoptosis. MEKK2 forms part of larger signaling complexes including the MAP3K complex where it regulates multiple cellular activities. Through its kinase activity MEKK2 passes on signals that affect gene expression and cellular stress responses impacting how cells react to their environment.
Pathways
MEKK2 functions prominently in the MAPK signaling and NF-kB pathways. These pathways involve signal transduction mechanisms that MEKK2 influences through its interactions with related proteins such as MEK5 and ERK5. By modulating these pathways MEKK2 participates in a variety of cellular responses including inflammation and immune responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Immunocytochemistry/ Immunofluorescence - Anti-MEKK2 antibody [EP626Y] (AB33918)](https://content.abcam.com/products/images/mekk2-antibody-ep626y-ab33918--immunocytochemistry-immunofluorescence-img108087.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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