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MAP4K3 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

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Images

Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956), expandable thumbnail
  • Sanger Sequencing - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956), expandable thumbnail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Alternative names

What's included?

1 Kit
Components
Human MAP4K3 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

MAP4K3 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Key facts

Cell type

HeLa

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

Concentration
Loading...

Properties

Gene name

MAP4K3

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Zygosity

Homozygous

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (ab258956), expandable thumbnail

    Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (ab258956)

    Lane 1: Wild-type HeLa cell lysate (20ug)

    Lane 2: MAP4K3 knockout HeLa cell lysate(20ug)

    Lane 3: HepG2 cell lysate (20ug)

    Lane 4: Daudi cell lysate (20ug)

    Lanes 1-2: Merged signal (red and green). Green - MAP4K3 (D1L4G) Rabbit monoclonal antibody observed at 100 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    MAP4K3 (D1L4G) Rabbit monoclonal antibody was shown to specifically react with MAP4K3 in wild-type HeLa cells in western blot. The band observed in the knockout cell line Human MAP4K3 (GLK) knockout HeLa cell line ab264796 (knockout cell lysate ab258956) lane below 100kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and MAP4K3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. MAP4K3 (D1L4G) Rabbit monoclonal antibody and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4oC at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: MAP4K3 (D1L4G) Rabbit mAb at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: MAP4K3 knockout HeLa cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Performed under reducing conditions.

  • Sanger Sequencing - Human MAP4K3 (GLK) knockout HeLa cell lysate (ab258956), expandable thumbnail

    Sanger Sequencing - Human MAP4K3 (GLK) knockout HeLa cell lysate (ab258956)

    Homozygous: Insertion of the selection cassette in exon 1

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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