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AB258956

Human MAP4K3 (GLK) knockout HeLa cell lysate

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Human MAP4K3 (GLK) knockout HeLa cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

Germinal center kinase like kinase, Germinal center kinase-related protein kinase, MAP4K3, MAPK/ERK kinase kinase kinase 3, MAPKKKK3, MEK kinase kinase 3, MEKKK 3, RAB8IPL1, mitogen-activated protein kinase kinase kinase kinase 3

2 Images
Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956)
  • WB

Lab

Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956)

Lane 1 : Wild-type HeLa cell lysate (20ug)

Lane 2 : MAP4K3 knockout HeLa cell lysate (20ug)

Lane 3 : HepG2 cell lysate (20ug)

Lane 4 : Daudi cell lysate (20ug)

Lanes 1-2 : Merged signal (red and green). Green - MAP4K3 (D1L4G) Rabbit monoclonal antibody observed at 100 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

MAP4K3 (D1L4G) Rabbit monoclonal antibody was shown to specifically react with MAP4K3 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264796 (knockout cell lysate ab258956) lane below 100kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and MAP4K3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. MAP4K3 (D1L4G) Rabbit monoclonal antibody and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

MAP4K3 (D1L4G) Rabbit mAb at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MAP4K3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MAP4K3 (GLK) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-map4k3-glk-knockout-hela-cell-line-ab264796'>ab264796</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

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Sanger Sequencing - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956)

Homozygous : Insertion of the selection cassette in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MAP4K3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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