AB257525

Human MAPK1 (ERK2) knockout HeLa cell lysate

Name: Human MAPK1 (ERK2) knockout HeLa cell lysate (ab257525) | Abcam
Brand: Abcam Limited
SKU: AB257525

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(1 Publication)

MAPK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

View Alternative Names

ERK-2, ERT1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MK01_HUMAN, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, P38, P41, PRKM 2, PRKM1, p42-MAPK, protein kinase, mitogen-activated, 1, protein kinase, mitogen-activated, 2, protein tyrosine kinase ERK2

5 Images

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Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : MAPK1 knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab32081 observed at 41 kDa. Red - loading control ab8245 observed at 37 kDa.

ab32081 Anti-ERK2 antibody [E460] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab32081 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/erk2-antibody-e460-ab32081'>ab32081</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MAPK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mapk1-erk2-knockout-hela-cell-line-ab265052'>ab265052</a>)

Predicted band size: 41 kDa

Observed band size: 41 kDa

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Lab

Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : MAPK1 knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab184699 observed at 44 kDa. Red - loading control ab8245 observed at 37 kDa.

ab184699 Anti-ERK1 + ERK2 antibody [EPR17526] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab184699 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (<a href='/en-us/products/primary-antibodies/erk1-erk2-antibody-epr17526-ab184699'>ab184699</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MAPK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mapk1-erk2-knockout-hela-cell-line-ab265052'>ab265052</a>)

Predicted band size: 41 kDa

Observed band size: 44 kDa

false

Lab

Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

All lanes:

Western blot - Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/erk2-antibody-e460-ab32081'>ab32081</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mapk1-erk2-knockout-hela-cell-line-ab265052'>ab265052</a>) at 20 µg

Observed band size: 41 kDa

false

Sanger Sequencing - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Sanger seq

Unknown

Sanger Sequencing - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Allele-1 : 1 bp deletion in exon 2

Sanger seq

Unknown

Sanger Sequencing - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Allele-2 : Insertion of the selection cassette in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

MAPK1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.

Biological function summary

ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.

Pathways

ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.

ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Apoptosis : an international journal on programmed cell death 27:800-811 PubMed36103025

2022

LINC00511 promotes cervical cancer progression by regulating the miR-497-5p/MAPK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Lu,Qing Gao,Yafei Wang,Jie Ren,Tingting Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MAPK1 (ERK2) knockout HeLa cell lysate

Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Western blot - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Sanger Sequencing - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Sanger Sequencing - Human MAPK1 (ERK2) knockout HeLa cell lysate (AB257525)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Publications (1)

Complementary Products

Cell Lines & Lysates

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise