MAPK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
HeLa
Human
Cervix
Sanger Sequencing, Western blot
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
ERK-2, ERT1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MK01_HUMAN, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, P38, P41, PRKM 2, PRKM1, p42-MAPK, protein kinase, mitogen-activated, 1, protein kinase, mitogen-activated, 2, protein tyrosine kinase ERK2
MAPK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
HeLa
Human
Cervix
Sanger Sequencing, Western blot
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Adenocarcinoma
MAPK1
Knockout
CRISPR technology
Sanger Sequencing, Western blot
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
EU: 2 US: 2
Adherent
Female
Ambient - Can Ship with Ice
-20°C
-20°C
Knockout cell lysate achieved by CRISPR/Cas9.
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Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.
ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.
ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.
ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.
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Lane 1: Wild-type HeLa cell lysate (20 μg)
Lane 2: MAPK1 knockout HeLa cell lysate (20 μg)
Lanes 1-2: Merged signal (red and green). Green - Anti-ERK1 + ERK2 antibody [EPR17526] ab184699 observed at 44 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-ERK1 + ERK2 antibody [EPR17526] ab184699 Anti-ERK1 + ERK2 antibody [EPR17526] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MAPK1 (ERK2) knockout HeLa cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. Anti-ERK1 + ERK2 antibody [EPR17526] ab184699 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 10000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (Anti-ERK1 + ERK2 antibody [EPR17526] ab184699) at 1/10000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MAPK1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 44 kDa
Lane 1: Wild-type HeLa cell lysate (20 μg)
Lane 2: MAPK1 knockout HeLa cell lysate (20 μg)
Lanes 1-2: Merged signal (red and green). Green - Anti-ERK2 antibody [E460] ab32081 observed at 41 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-ERK2 antibody [E460] ab32081 Anti-ERK2 antibody [E460] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MAPK1 (ERK2) knockout HeLa cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. Anti-ERK2 antibody [E460] ab32081 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MAPK1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution
Lane 1: Wild-type HeLa lysate at 20 µg
Lane 2: Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (Human MAPK1 (ERK2) knockout HeLa cell line ab265052) at 20 µg
Observed band size: 41 kDa
Allele-1: 1 bp deletion in exon 2
Allele-2: Insertion of the selection cassette in exon 2
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