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AB263787

Human MAPK14 (p38) knockout HEK-293T cell lysate

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MAPK14 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1.

View Alternative Names

CSAID Binding Protein 1, CSAID-binding protein, CSBP, CSBP 1, CSBP 2, CSPB1, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, MK14_HUMAN, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, Mxi 2, P38, PRKM14, PRKM15, RK, SAPK2A, Stress-activated protein kinase 2a, p38 ALPHA, p38 MAP kinase, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip

3 Images
Western blot - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)
  • WB

Supplier Data

Western blot - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)

Lane 1 : HeLa cell lysate (20 μg)

Lane 2 : Jurkat cell lysate (20 μg)

Lane 3 : Wild-type HEK-293TT cell lysate (20 μg)

Lane 4 : MAPK14 knockout HEK-293TT cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab182453 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab182453 was shown to react with p38 in HEK-293TT wildtype. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab182453 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-p38 alpha/MAPK14 antibody [EPR16878] (<a href='/en-us/products/primary-antibodies/p38-alpha-mapk14-antibody-epr16878-ab182453'>ab182453</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK14 (p38) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mapk14-p38-knockout-hek-293t-cell-line-ab255406'>ab255406</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

MAPK14 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 124 kDa,38 kDa

false

Western blot - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)
  • WB

Lab

Western blot - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)

Lane 1 : HeLa cell lysate (20 μg)

Lane 2 : Jurkat cell lysate (20 μg)

Lane 3 : Wild-type HEK-293T cell lysate (20 μg)

Lane 4 : MAPK14 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (<a href='/en-us/products/primary-antibodies/p38-beta-mapk11-p38-alpha-mapk14-antibody-y122-ab32142'>ab32142</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK14 (p38) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mapk14-p38-knockout-hek-293t-cell-line-ab255406'>ab255406</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

MAPK14 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 124 kDa,42 kDa

false

Sanger Sequencing - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAPK14 (p38) knockout HEK-293T cell lysate (AB263787)

Allele-1 : 1 bp insertion in exon 1; Allele-2 : 11 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263787-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human MAPK14 knockout HEK293T cell lysate", "number":"AB263787-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263787-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
MAPK14
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P38 alpha also known as MAPK14 is a significant member of the MAP kinase family involved in cellular response to stress signals. This protein has a molecular mass of about 38 kDa and is expressed in various tissues throughout the body. p38 alpha plays an important role in the signal transduction pathways that regulate inflammatory responses and cell differentiation. Its activity is modulated by multiple upstream kinases leading to cell-specific effects that are important for organismal homeostasis.
Biological function summary

P38 alpha MAPK14 is a part of a larger mitogen-activated protein kinase (MAPK) complex where it serves to mediate signals from external stressors to the appropriate cellular processes. It is particularly active in its roles involving inflammation and apoptosis regulation. The protein interacts with other members of the MAPK family and additional proteins such as TAB1 to conduct these biological signals efficiently.

Pathways

P38 alpha integrates into the p38 MAPK pathway and the NF-kB signaling pathway which are essential for managing cellular stress responses and inflammatory reactions. It closely interacts with other proteins like MKK3 and MKK6 which are directly upstream regulators phosphorylating and activating p38 MAPK14. This intricate connection allows p38 alpha to execute precise regulation within cellular environments.

P38 alpha MAPK14 is prominently associated with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. In these conditions its aberrant activation or expression can lead to pathological inflammation and tissue damage. Additionally p38 alpha’s connection with TNF-alpha in inflammation highlights its relevance in therapeutic targets for related disorders reflecting the significance of its modulation to potentially mitigate disease progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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