Human MAPK7 (ERK5) knockout HeLa cell lysate
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MAPK7 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
View Alternative Names
BMK 1 kinase, BMK-1, Big MAP kinase 1, EC 2.7.11.24, ERK-4, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MK07_HUMAN, Mitogen-activated protein kinase 7, OTTHUMP00000065906, OTTHUMP00000065907, PRKM 7, PROTEIN KINASE, MITOGEN-ACTIVATED, 7
- WB
Lab
Western blot - Human MAPK7 (ERK5) knockout HeLa cell lysate (AB258042)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : MAPK7 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab40809 observed at 115 kDa. Red - loading control ab8245 observed at 37 kDa.
ab40809 Rabbit monoclonal [EP791Y] to ERK5 was shown to specifically react with ERK5 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265508 (knockout cell lysate ab258042) was used. Wild-type and ERK5 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab40809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ERK5 antibody [EP791Y] (<a href='/en-us/products/primary-antibodies/erk5-antibody-ep791y-ab40809'>ab40809</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
MAPK7 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human MAPK7 (ERK5) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mapk7-erk5-knockout-hela-cell-line-ab265508'>ab265508</a>)
Predicted band size: 88 kDa
Observed band size: 115 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human MAPK7 (ERK5) knockout HeLa cell lysate (AB258042)
Homozygous : 1 bp insertion in exon4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.
Pathways
ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Flow Cytometry (Intracellular) - Anti-ERK5 antibody [EP791Y] (AB40809)](https://content.abcam.com/products/images/erk5-antibody-ep791y-ab40809--flow-cytometry-intracellular-img140109.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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