MAT2B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
Alternative names
1110064C04Rik, 2410018D16Rik, AI182287, AU022853, Beta regulatory subunit of methionine adenosyltransferase, DTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT II beta, MAT-II, MAT2B_HUMAN, MGC12237, MSTP045, Methionine adenosyltransferase 2 beta subunit, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Nbla02999, OTTMUSP00000005600, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Putative protein product of Nbla02999, RP23-382C18.2, SDR23E1, Short chain dehydrogenase/reductase family 23E member 1, TGR
What's included?
Recommended products
MAT2B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
- Concentration
Properties
- Gene name
- MAT2B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAT2B also known as TGR is a protein that functions as a regulatory subunit of the enzyme methionine adenosyltransferase II. This enzyme synthesizes S-adenosylmethionine (SAM) essential for methylation reactions in varied cellular processes. MAT2B has a molecular mass of approximately 35 kDa and can modulate the activity of the catalytic subunit of the methionine adenosyltransferase complex. The protein is expressed in multiple tissues with abundant levels in liver and fetal tissues. It is important for regulating the levels of SAM linking dietary methionine to cellular methylation capacity.
- Biological function summary
The MAT2B protein helps maintain cellular methylation status by adjusting SAM synthesis. It is a part of the methionine adenosyltransferase II complex which consists of different subunits contributing to its enzymatic activity. The regulatory role of MAT2B ensures precise control over the methionine cycle impacting DNA methylation patterns and gene regulation. By modulating SAM production MAT2B influences cellular growth differentiation and apoptosis through its interactions with other methylation-related proteins.
- Pathways
The MAT2B protein plays a significant role in methionine metabolism and the transmethylation pathway. These pathways are central to transferring methyl groups from SAM to a range of substrates including DNA proteins and lipids. MAT2B is related to MAT2A the catalytic subunit that generates SAM. Together they coordinate to balance cellular metabolic needs with the synthesis and utilization of methyl groups securing the methylation-dependent regulation of diverse cellular processes.
- Associated diseases and disorders
MAT2B associates with liver diseases and cancer. Its regulatory function in SAM synthesis ties it to disruptions in methylation capacity observed in these conditions. Reduced MAT2B activity can lead to imbalanced SAM levels affecting the methylation landscape and contributing to altered gene expression involved in tumorigenesis. In liver diseases MAT2B interacts with MAT1A a protein facilitating methylation balance in mature liver tissue. This interaction highlights MAT2B's significant impact on maintaining liver health and its potential role in pathogenesis when dysregulated.
Product promise
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2 product images
Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (ab258044)
Allele-2: 5 bp deletion in exon 1
Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (ab258044)
Allele-1: 16 bp deletion in exon 1
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Product protocols
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