Human MAT2B (TGR) knockout HEK-293T cell lysate
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MAT2B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
View Alternative Names
1110064C04Rik, 2410018D16Rik, AI182287, AU022853, Beta regulatory subunit of methionine adenosyltransferase, DTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT II beta, MAT-II, MAT2B_HUMAN, MGC12237, MSTP045, Methionine adenosyltransferase 2 beta subunit, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Nbla02999, OTTMUSP00000005600, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Putative protein product of Nbla02999, RP23-382C18.2, SDR23E1, Short chain dehydrogenase/reductase family 23E member 1, TGR
- Sanger seq
Unknown
Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)
Allele-1 : 16 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)
Allele-2 : 5 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The MAT2B protein helps maintain cellular methylation status by adjusting SAM synthesis. It is a part of the methionine adenosyltransferase II complex which consists of different subunits contributing to its enzymatic activity. The regulatory role of MAT2B ensures precise control over the methionine cycle impacting DNA methylation patterns and gene regulation. By modulating SAM production MAT2B influences cellular growth differentiation and apoptosis through its interactions with other methylation-related proteins.
Pathways
The MAT2B protein plays a significant role in methionine metabolism and the transmethylation pathway. These pathways are central to transferring methyl groups from SAM to a range of substrates including DNA proteins and lipids. MAT2B is related to MAT2A the catalytic subunit that generates SAM. Together they coordinate to balance cellular metabolic needs with the synthesis and utilization of methyl groups securing the methylation-dependent regulation of diverse cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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