Human MAVS knockout A549 cell lysate

• WB

Lab

Western blot - Human MAVS knockout A549 cell lysate (AB283026)

False colour image of Western blot : Anti-MAVS antibody [abM28C8] staining at 2 ug/ml shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution shown in red. In Western blot ab220170 was shown to bind specifically to MAVS. A band was observed at 70 kDa in wild-type A549 cell lysates with no signal observed at this size in Mavs knockout cell line ab282354 (knockout cell lysate ab283026). To generate this image wild-type and Mavs knockout A549 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-MAVS antibody [ABM28C8] (<a href='/en-us/products/primary-antibodies/mavs-antibody-abm28c8-ab220170'>ab220170</a>) at 2 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Mavs knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human MAVS knockout A549 cell line (<a href='/en-us/products/cell-lines/human-mavs-knockout-a549-cell-line-ab282354'>ab282354</a>)

Lane 2:

Western blot - Human MAVS knockout A549 cell lysate (ab283026)

Predicted band size: 57 kDa

Observed band size: 70 kDa

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• WB

Lab

Western blot - Human MAVS knockout A549 cell lysate (AB283026)

False colour image of Western blot : Anti-MAVS antibody [ABM28C8] staining at 2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220170 was shown to bind specifically to MAVS. A band was observed at 70 kDa in wild-type A549 cell lysates with no signal observed at this size in Mavs knockout cell line ab282354 (knockout cell lysate ab283026). To generate this image, wild-type and Mavs knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

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• Sanger seq

Supplier Data

Sanger Sequencing - Human MAVS knockout A549 cell lysate (AB283026)

101 bp deletion in exon 5