Human MBD4 (MED1) knockout HeLa cell lysate
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MBD4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
View Alternative Names
3 N(4) ethenocytosine glycosylase, G/5 fluorouracil mismatch glycosylase with biphasic kinetics, G/T mismatch glycosylase, G/U mismatch glycosylase, MBD4_HUMAN, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, Putative methyl CpG binding protein
- Sanger seq
Unknown
Sanger Sequencing - Human MBD4 (MED1) knockout HeLa cell lysate (AB258960)
Homozygous : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MBD4 participates in the protection of genome integrity. It is involved in the DNA demethylation process ensuring proper gene expression patterns. MBD4 functions as part of complex DNA repair machinery. By working with other repair proteins MBD4 facilitates the correction of errors arising during DNA replication and demethylation reducing the risk of mutation accumulation and genomic instability.
Pathways
MBD4 plays a significant role in the base excision repair (BER) pathway. This pathway is key for correcting DNA damage caused by deamination and other forms of DNA alteration. In the BER pathway MBD4 cooperates with proteins like DNA glycosylases to identify and remove incorrect bases from the DNA strand. MBD4 also interacts with transcriptional regulation pathways influencing how genes are expressed in response to changes in the epigenetic landscape.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB315462
Anti-MBD4/MED1 antibody [EPR28263-16]
20ul selling size|RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-MBD4/MED1 antibody [EPR28263-16] (AB315462)](https://content.abcam.com/products/images/mbd4-med1-antibody-epr28263-16-ab315462--flow-cytometry-intracellular-img426169.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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